Optimization of the immobilization of bacterial spores on glass substrates with organosilanes

被引：4

作者：

Arreola, Julio ^{[1
]}

Maetzkow, Malte ^{[1
]}

Duran, Marlena Palomar ^{[2
]}

Greeff, Anton ^{[2
]}

Keusgen, Michael ^{[3
]}

Schoening, Michael J. ^{[1
]}

机构：

[1] Aachen Univ Appl Sci, Inst Nano & Biotechnol, Heinrich Mussmann Str 1, D-52428 Julich, Germany

[2] Elopak EQS GmbH, Hanns Martin Schleyer Str 17, D-41199 Monchengladbach, Germany

[3] Univ Marburg, Inst Pharmaceut Chem, Marbacher Weg 6-10, D-35032 Marburg, Germany

来源：

PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE | 2016年 / 213卷 / 06期

关键词：

Bacillus atrophaeus; biosensors; endospores; immobilization; organosilanes; silanization; SELF-ASSEMBLED MONOLAYERS; BACILLUS-SUBTILIS; ALKYLSILOXANE MONOLAYERS; SURFACES; SILICA; SILANIZATION; FILMS; OLIGONUCLEOTIDES; RESISTANCE; STABILITY;

D O I：

10.1002/pssa.201532914

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Spores can be immobilized on biosensors to function as sensitive recognition elements. However, the immobilization can affect the sensitivity and reproducibility of the sensor signal. In this work, three different immobilization strategies with organosilanes were optimized and characterized to immobilize Bacillus atrophaeus spores on glass substrates. Five different silanization parameters were investigated: nature of the solvent, concentration of the silane, silanization time, curing process, and silanization temperature. The resulting silane layers were resistant to a buffer solution (e.g., Ringer solution) with a polysorbate (e.g., Tween (R) 1 80) and sonication. [GRAPHICS] AFM image of silanization on a glass substrate with an organosilane (e.g., APTES). (C) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：1463 / 1470

页数：8

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