Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases

被引：43

作者：

Sakakibara, Y ^{[1
]}

Yanagisawa, K

Takami, Y

Nakayama, T

Suiko, M

Liu, MC

机构：

[1] Univ Texas, Ctr Hlth, Dept Biochem, Tyler, TX 75710 USA

[2] Miyazaki Med Coll, Dept Biochem, Miyazaki 88916, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 247卷 / 03期

基金：

日本学术振兴会;

关键词：

D O I：

10.1006/bbrc.1998.8872

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleotide sequences of two mouse cDNAs encoding new sulfotransferase enzymes were determined. Analysis of the deduced amino acid sequences revealed that one represents a novel member of the phenol sulfotransferase family and the other is highly homologous to human SULT2B1 hydroxysteroid sulfotransferases. The recombinant enzymes, transiently expressed in COS-7 cells, were characterized with respect to their substrate specificity using a variety of substrates for different types of sulfotransferases. The tissue-specific expression of these two new mouse sulfotransferases was examined by Northern blot analysis. (C) 1998 Academic Press.

引用

页码：681 / 686

页数：6

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