Identification of alternatively spliced isoforms of interleukin-2/15 receptor β chain in ducks

被引：5
|
作者：
Jeong, Jipseol [1 ,2 ,3 ]
Kim, Woo H. [1 ,2 ]
Yeo, Jaeseung [1 ,2 ]
Fernandez, Cherry P. [1 ,2 ]
Kim, Suk [1 ,2 ]
Lee, Youn-Jeong [3 ]
Lillehoj, Hyun S. [4 ]
Min, Wongi [1 ,2 ]
机构：
[1] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, Gyeongnam, South Korea
[2] Gyeongsang Natl Univ, Inst Anim Med, Jinju 660701, Gyeongnam, South Korea
[3] Anim & Plant Quarantine Agcy, Avian Dis Div, Anyang 430757, South Korea
[4] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA
来源：
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2014年 / 162卷 / 3-4期
关键词：
Duck; IL-2/1512; beta; Isoforms; Riemerella anatipestifer infection; gene expression; MICE LACKING; IL-2; EXPRESSION; ASSOCIATION; ACTIVATION; JAK1;
D O I：
10.1016/j.vetimm.2014.09.005
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Interleukin (IL)-2 and IL-15 receptor 13 (IL-2/15R beta, CD122) play important roles in signal transduction for biological functions of IL-2 and IL-15. We found that ducks possess three different IL-2/15R beta transcripts, a conventional form (duIL-2/15R beta) and two variants. Comparisons between the cDNA and genomic sequences revealed that the two variants, duIL-2/15R beta-d7 and duIL-2/15R beta-d9, were novel spliced transcripts resulting from skipping exons 7 and 9, respectively. Expression profiles of duIL-2/15R beta and its isoforms were examined in healthy tissues, concanavalin A (ConA)-stimulated splenic lymphocytes and in livers and spleens of Riemerella anatipestifer-infected ducks using quantitative realtime PCR (qRT-PCR). Generally, duIL-2/15R beta-d9 expression was undetectable in healthy tissues, ConA-activated samples, and R. anatipestifer-infected ducks. Expression levels of duIL-2/15R beta transcript were relatively high to moderate in all healthy tissues tested, while duIL-2/15R13-d7 expression was low. Compared to untreated controls, expression levels of duIL-2/15R beta were elevated in ConA-activated splenic lymphocytes and in livers on day 7 in R. anatipestifer-infected ducks, while duIL-2/15R beta-d7 expression was unchanged. Additionally, COS-7 cells transfected with duIL-2/15R beta, duIL-2/15R beta-d7, or duIL-2/15R beta-d9 constructs generated 73 kilodalton (kDa), 31 kDa, and 40 kDa proteins, respectively. This study identified three different IL-2/15R beta transcripts, including two isoforms generated by alternative splicing and their gene expression patterns in stimulated conditions. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：154 / 161
页数：8
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