Identification of alternatively spliced isoforms of interleukin-2/15 receptor β chain in ducks

被引：5
|
作者：
Jeong, Jipseol [1 ,2 ,3 ]
Kim, Woo H. [1 ,2 ]
Yeo, Jaeseung [1 ,2 ]
Fernandez, Cherry P. [1 ,2 ]
Kim, Suk [1 ,2 ]
Lee, Youn-Jeong [3 ]
Lillehoj, Hyun S. [4 ]
Min, Wongi [1 ,2 ]
机构：
[1] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, Gyeongnam, South Korea
[2] Gyeongsang Natl Univ, Inst Anim Med, Jinju 660701, Gyeongnam, South Korea
[3] Anim & Plant Quarantine Agcy, Avian Dis Div, Anyang 430757, South Korea
[4] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA
来源：
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2014年 / 162卷 / 3-4期
关键词：
Duck; IL-2/1512; beta; Isoforms; Riemerella anatipestifer infection; gene expression; MICE LACKING; IL-2; EXPRESSION; ASSOCIATION; ACTIVATION; JAK1;
D O I：
10.1016/j.vetimm.2014.09.005
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Interleukin (IL)-2 and IL-15 receptor 13 (IL-2/15R beta, CD122) play important roles in signal transduction for biological functions of IL-2 and IL-15. We found that ducks possess three different IL-2/15R beta transcripts, a conventional form (duIL-2/15R beta) and two variants. Comparisons between the cDNA and genomic sequences revealed that the two variants, duIL-2/15R beta-d7 and duIL-2/15R beta-d9, were novel spliced transcripts resulting from skipping exons 7 and 9, respectively. Expression profiles of duIL-2/15R beta and its isoforms were examined in healthy tissues, concanavalin A (ConA)-stimulated splenic lymphocytes and in livers and spleens of Riemerella anatipestifer-infected ducks using quantitative realtime PCR (qRT-PCR). Generally, duIL-2/15R beta-d9 expression was undetectable in healthy tissues, ConA-activated samples, and R. anatipestifer-infected ducks. Expression levels of duIL-2/15R beta transcript were relatively high to moderate in all healthy tissues tested, while duIL-2/15R13-d7 expression was low. Compared to untreated controls, expression levels of duIL-2/15R beta were elevated in ConA-activated splenic lymphocytes and in livers on day 7 in R. anatipestifer-infected ducks, while duIL-2/15R beta-d7 expression was unchanged. Additionally, COS-7 cells transfected with duIL-2/15R beta, duIL-2/15R beta-d7, or duIL-2/15R beta-d9 constructs generated 73 kilodalton (kDa), 31 kDa, and 40 kDa proteins, respectively. This study identified three different IL-2/15R beta transcripts, including two isoforms generated by alternative splicing and their gene expression patterns in stimulated conditions. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：154 / 161
页数：8
相关论文
共 50 条
  • [1] Identification and characterization of three new alternatively spliced μ-opioid receptor isoforms
    Pan, YX
    Xu, J
    Bolan, E
    Abbadie, C
    Chang, A
    Zuckerman, A
    Rossi, G
    Pasternak, GW
    MOLECULAR PHARMACOLOGY, 1999, 56 (02) : 396 - 403
  • [2] ALTERNATIVELY SPLICED ISOFORMS OF THE NMDARI RECEPTOR SUBUNIT
    ZUKIN, RS
    BENNETT, MVL
    TRENDS IN NEUROSCIENCES, 1995, 18 (07) : 306 - 313
  • [3] Identification and Characterization of the Alternatively Spliced Nuclear Receptor Coactivator-6 Isoforms
    Li, Qingtian
    Xu, Jianming
    INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2011, 7 (05): : 505 - 516
  • [4] Alternatively spliced isoforms of the human constitutive androstane receptor
    Auerbach, SS
    Ramsden, R
    Stoner, MA
    Verlinde, C
    Hassett, C
    Omiecinski, CJ
    NUCLEIC ACIDS RESEARCH, 2003, 31 (12) : 3194 - 3207
  • [5] Identification of an alternatively spliced isoform of the common cytokine receptor γ chain in chickens
    Min, WG
    Lillehoj, HS
    Fetterer, RH
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 299 (02) : 321 - 327
  • [6] Human amniotic fluid lacks interleukin-2 and interleukin-15 but can interact with the β-chain of the interleukin-2 receptor
    Searle, RF
    Bromage, SJ
    Palmer, J
    Curry, JE
    Lang, AK
    IMMUNOLOGY, 2000, 99 (03) : 411 - 417
  • [7] Identification of alternatively spliced dab1 isoforms in zebrafish
    Arianna Costagli
    Barbara Felice
    Alessandro Guffanti
    Stephen W. Wilson
    Marina Mione
    Development Genes and Evolution, 2006, 216 : 291 - 299
  • [8] Analysis of alternatively spliced isoforms of human LDL receptor mRNA
    Tveten, Kristian
    Ranheim, Trine
    Berge, Knut Erik
    Leren, Trond P.
    Kulseth, Mari Ann
    CLINICA CHIMICA ACTA, 2006, 373 (1-2) : 151 - 157
  • [9] Identification of alternatively spliced dab1 isoforms in zebrafish
    Costagli, Arianna
    Felice, Barbara
    Guffanti, Alessandro
    Wilson, Stephen W.
    Mione, Marina
    DEVELOPMENT GENES AND EVOLUTION, 2006, 216 (06) : 291 - 299
  • [10] Characterization of the human β-glucan receptor and its alternatively spliced isoforms
    Willment, JA
    Gordon, S
    Brown, GD
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (47) : 43818 - 43823
12345