Label-free detection for SNP using AIE probes and carbon nanotubes

被引:27
作者
Ma, Ke [1 ]
Wang, Hui [1 ]
Li, Haolong [1 ]
Xu, Bin [1 ]
Tian, Wenjing [1 ]
机构
[1] Jilin Univ, State Key Lab Supramol Struct & Mat, 2699 Qianjin St, Changchun 130012, Jilin, Peoples R China
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 253卷
基金
高等学校博士学科点专项科研基金;
关键词
SNP analysis; AIE probe; Label-free detection; DNA; Water-soluble CNTs; AGGREGATION-INDUCED EMISSION; SINGLE-NUCLEOTIDE POLYMORPHISM; ON FLUORESCENT-PROBE; CIRCULAR-DICHROISM; DNA; APTASENSOR; MOLECULES; GENE; TAU;
D O I
10.1016/j.snb.2017.06.055
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We design a label-free optical platform for SNP detection by using aggregation-induced emission (AIE) active molecule DSAI as the probe and water-soluble carbon nanotubes as the selective platform via the fluorescent quenching effect. In the presence of DNA, DSAI molecules aggregate on DNA through the intercalation and the electrostatic interaction as well as the hydrophobic interaction, resulting in the strong fluorescence in the solution. In order to distinguish the wild-DNA from the single nucleotide mutated-DNA, we induce the water-soluble carbon nanotubes as the effective fluorescent quenching material. The interactions between DSAI and wild-DNA are rather strong, thus DSAI molecules cannot be hauled out from wild-DNA and the fluorescence stays still. While the interactions between DSAI and single nucleotide mutated-DNA are relatively weak, and several DSAI molecules can be hauled out from the single nucleotide mutated-DNA by the water-soluble carbon nanotubes through the strong electrostatic interaction, leading to the partial fluorescent quenching in the solution. Therefore, the detection of SNP in the random DNA sequence could be realized by using AIE probes and carbon nanotubes. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:92 / 96
页数:5
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