A new method for detection of pandemic influenza virus using High Resolution Melting analysis of the neuraminidase gene

被引：17

作者：

Varillas, David ^{[1
,2
]}

Bermejo-Martin, Jesus F. ^{[1
,2
]}

Almansa, Raquel ^{[1
,2
]}

Rojo, Silvia ^{[1
]}

Nogueira, Begona ^{[1
]}

Maria Eiros, Jose ^{[1
]}

Rico, Lucia ^{[1
,2
]}

Iglesias, Veronica ^{[1
,2
]}

Ortiz de Lejarazu, Raul ^{[1
,2
]}

机构：

[1] Natl Influenza Ctr, Valladolid, Spain

[2] Clin Univ Hosp Valladolid IECSCYL, Infect & Immun Unit, Microbiol Serv, Valladolid, Spain

来源：

JOURNAL OF VIROLOGICAL METHODS | 2011年 / 171卷 / 01期

关键词：

HRM; Influenza; Neuraminidase; RT-PCR; IDENTIFICATION; VARIANTS; PCR;

D O I：

10.1016/j.jviromet.2010.10.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Diagnostic methods based upon exclusive detection of haemagglutinin do not detect sequence variation in other gene segments of the Influenza A virus. A complementary approach is described based upon high-resolution melting curve analysis of the neuraminidase gene, an approach with the potential ability to detect small changes in the neuraminidase sequence without the need for specific probes. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：284 / 286

页数：3

共 9 条

[1] Rapid detection and identification of clinically important bacteria by high-resolution melting analysis after broad-range ribosomal RNA real-time PCR [J].

Cheng, Ju-Chien ;

Huang, Chien-Ling ;

Lin, Chung-Ching ;

Chen, Chi-Ching ;

Chang, Yi-Chih ;

Chang, Shy-Shin ;

Tseng, Ching-Ping .

CLINICAL CHEMISTRY, 2006, 52 (11) :1997-2004

[2] Optimization of high-resolution melting analysis for low-cost and rapid screening of allelic variants of Bacillus anthracis by multiple-locus variable-number tandem repeat analysis [J].

Fortini, Daniela ;

Ciammaruconi, Andrea ;

De Santis, Riccardo ;

Fasanella, Antonio ;

Battisti, Antonio ;

D'Amelio, Raffaele ;

Lista, Florigio ;

Cassone, Antonio ;

Carattoli, Alessandra .

CLINICAL CHEMISTRY, 2007, 53 (07) :1377-1380

[3] Emergence and spread of oseltamivir-resistant A(H1N1) influenza viruses in Oceania, South East Asia and South Africa [J].

Hurt, Aeron C. ;

Ernest, Joanne ;

Deng, Yi-Mo ;

Iannello, Pina ;

Besselaar, Terry G. ;

Birch, Chris ;

Buchy, Philippe ;

Chittaganpitch, Malinee ;

Chiu, Shu-Chun ;

Dwyer, Dominic ;

Guigon, Aurelie ;

Harrower, Bruce ;

Kei, Ip Peng ;

Kok, Tuckweng ;

Lin, Cui ;

McPhie, Ken ;

Mohd, Apandi ;

Olveda, Remigio ;

Panayotou, Tony ;

Rawlinson, William ;

Scott, Lesley ;

Smith, David ;

D'Souza, Holly ;

Komadina, Naomi ;

Shaw, Robert ;

Kelso, Anne ;

Barr, Ian G. .

ANTIVIRAL RESEARCH, 2009, 83 (01) :90-93

[4] Classification of Mycoplasma synoviae strains using single-strand conformation polymorphism and high-resolution melting-curve analysis of the vIhA gene single-copy region [J].

Jeffery, Nathan ;

Gasser, Robin B. ;

Steer, Penelope A. ;

Noormohammadi, Amir H. .

MICROBIOLOGY-SGM, 2007, 153 :2679-2688

[5] Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons [J].

Liew, M ;

Pryor, R ;

Palais, R ;

Meadows, C ;

Erali, M ;

Lyon, E ;

Wittwer, C .

CLINICAL CHEMISTRY, 2004, 50 (07) :1156-1164

[6] Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis [J].

Lin, Jih-Hui ;

Tseng, Ching-Ping ;

Chen, Yen-Ju ;

Lin, Chy-Yung ;

Shin-Yang, Shy ;

Wu, Ho-Sheng ;

Cheng, Ju-Chien .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (03) :1090-1097

[7]

Odell ID, 2005, AM J CLIN PATHOL, V123, P96, DOI 10.1309/WDR082X9FFJBQQGB

[8]

*WHO, 2010, EP PAND AL RESP INFL

[9] High-resolution genotyping by amplicon melting analysis using LCGreen [J].

Wittwer, CT ;

Reed, GH ;

Gundry, CN ;

Vandersteen, JG ;

Pryor, RJ .

CLINICAL CHEMISTRY, 2003, 49 (06) :853-860

← 1 →