Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer

被引：70

作者：

Simonetti, Sara ^{[1
]}

Angel Molina, Miguel ^{[1
]}

Queralt, Cristina ^{[2
]}

de Aguirre, Itziar ^{[2
]}

Mayo, Clara ^{[1
]}

Bertran-Alamillo, Jordi ^{[1
]}

Javier Sanchez, Jose ^{[3
]}

Luis Gonzalez-Larriba, Jose ^{[4
]}

Jimenez, Ulpiano ^{[5
]}

Isla, Dolores ^{[6
]}

Moran, Teresa ^{[2
]}

Viteri, Santiago ^{[1
]}

Camps, Carlos ^{[7
]}

Garcia-Campelo, Rosario ^{[8
]}

Massuti, Bartomeu ^{[9
]}

Benlloch, Susana ^{[1
]}

Ramon y Cajal, Santiago ^{[1
,10
]}

Taron, Miquel ^{[1
,2
]}

Rosell, Rafael ^{[1
,2
]}

机构：

[1] USP Dexeus Univ Inst, Barcelona, Spain

[2] Hosp Badalona Germans Trias & Pujol, Catalan Inst Oncol, Barcelona, Spain

[3] Autonomous Univ Madrid, E-28049 Madrid, Spain

[4] Hosp San Carlos, Madrid, Spain

[5] Hosp La Princesa, Madrid, Spain

[6] Hosp Lozano Blesa, Zaragoza, Spain

[7] Hosp Gen Valencia, Valencia, Spain

[8] Hosp Juan Canalejo, La Coruna, Spain

[9] Hosp Gen Alicante, Alicante, Spain

[10] Hosp Valle De Hebron, Barcelona, Spain

来源：

JOURNAL OF TRANSLATIONAL MEDICINE | 2010年 / 8卷

关键词：

FACTOR-RECEPTOR GENE; SENSITIVE METHOD; CLINICAL-RESPONSE; ADENOCARCINOMA; EXPRESSION; DIAGNOSIS;

D O I：

10.1186/1479-5876-8-135

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background: Immunohistochemistry (IHC) with mutation-specific antibodies may be an ancillary method of detecting EGFR mutations in lung cancer patients. Methods: EGFR mutation status was analyzed by DNA assays, and compared with IHC results in five non-small-cell lung cancer (NSCLC) cell lines and tumor samples from 78 stage IV NSCLC patients. Results: IHC correctly identified del 19 in the H1650 and PC9 cell lines, L858R in H1975, and wild-type EGFR in H460 and A549, as well as wild-type EGFR in tumor samples from 22 patients. IHC with the mAb against EGFR with del 19 was highly positive for the protein in all 17 patients with a 15-bp (ELREA) deletion in exon 19, whereas in patients with other deletions, IHC was weakly positive in 3 cases and negative in 9 cases. IHC with the mAb against the L858R mutation showed high positivity for the protein in 25/27 (93%) patients with exon 21 EGFR mutations (all with L858R) but did not identify the L861Q mutation in the remaining two patients. Conclusions: IHC with mutation-specific mAbs against EGFR is a promising method for detecting EGFR mutations in NSCLC patients. However these mAbs should be validated with additional studies to clarify their possible role in routine clinical practice for screening EGFR mutations in NSCLC patients.

引用

页数：8

共 23 条

[1] Assessment of EGFR Mutation Status in Lung Adenocarcinoma by Immunohistochemistry Using Antibodies Specific to the Two Major Forms of Mutant EGFR [J].