PhaseRMiC: phase real-time microscope camera for live cell imaging

被引：15

作者：

Chen, Chao ^{[1
]}

Lu, Yu-Nan ^{[2
]}

Huang, Huachuan ^{[3
]}

Yan, Keding ^{[4
]}

Jiang, Zhilong ^{[1
]}

He, Xiaoliang ^{[1
]}

Kong, Yan ^{[1
]}

Liu, Cheng ^{[1
,5
]}

Liu, Fei ^{[2
]}

Xue, Liang ^{[6
]}

Wang, Shouyu ^{[1
,2
]}

机构：

[1] Jiangnan Univ, Sch Sci, Computat Opt Lab, Wuxi 214122, Jiangsu, Peoples R China

[2] Nanjing Agr Univ, Single Mol Nanometry Lab Sinmolab, Nanjing 210095, Peoples R China

[3] Southwest Univ Sci & Technol, Sch Manufacture Sci & Engn, Key Lab Testing Technol Mfg Proc, Minist Educ, Mianyang 621010, Sichuan, Peoples R China

[4] Xian Technol Univ, Sch Elect Informat Engn, Adv Inst Micronano Intelligent Sensing AIMNIS, Xian 710032, Shaanxi, Peoples R China

[5] Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China

[6] Shanghai Univ Elect Power, Coll Elect & Informat Engn, Shanghai 200090, Peoples R China

来源：

BIOMEDICAL OPTICS EXPRESS | 2021年 / 12卷 / 08期

基金：

中国国家自然科学基金;

关键词：

LATERAL SHEARING INTERFEROMETRY; FLIPPING INTERFEROMETRY; INTENSITY EQUATION; TRANSPORT; FIELD; DYNAMICS; COMPACT;

D O I：

10.1364/BOE.430115

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We design a novel phase real-time microscope camera (PhaseRMiC) for live cell phase imaging. PhaseRMiC has a simple and cost-effective configuration only consisting of a beam splitter and a board-level camera with two CMOS imaging chips. Moreover, integrated with 3-D printed structures, PhaseRMiC has a compact size of 136x91x60 mm(3), comparable to many commercial microscope cameras, and can be directly connected to the microscope side port. Additionally, PhaseRMiC can be well adopted in real-time phase imaging proved with satisfied accuracy, good stability and large field of view. Considering its compact and cost-effective device design as well as real-time phase imaging capability, PhaseRMiC is a preferred solution for live cell imaging. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：5261 / 5271

页数：11

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