Noninvasive Diagnosis and Molecular Phenotyping of Breast Cancer through Microbead-Assisted Flow Cytometry Detection of Tumor-Derived Extracellular Vesicles

被引：33

作者：

Li, Wenzhe ^{[1
,2
]}

Shao, Bin ^{[3
]}

Liu, Changliang ^{[1
]}

Wang, Huayi ^{[1
]}

Zheng, Wangshu ^{[1
]}

Kong, Weiyao ^{[3
]}

Liu, Xiaoran ^{[3
]}

Xu, Guobin ^{[4
]}

Wang, Chen ^{[1
]}

Li, Huiping ^{[3
]}

Zhu, Ling ^{[1
]}

Yang, Yanlian ^{[1
]}

机构：

[1] Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, CAS Key Lab Standardizat & Measurement Nanotechno, Beijing 100190, Peoples R China

[2] Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China

[3] Peking Univ, Canc Hosp & Inst, Dept Breast Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China

[4] Peking Univ, Canc Hosp & Inst, Clin Lab, Beijing 100142, Peoples R China

来源：

SMALL METHODS | 2018年 / 2卷 / 11期

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

breast cancer; detection; extracellular vesicles; phenotyping; EPITHELIAL-MESENCHYMAL TRANSITION; INTRATUMOR HETEROGENEITY; EXOSOMAL MICRORNA; AMERICAN-SOCIETY; HER2; EXPRESSION; LYMPH-NODE; BLOOD; EPCAM; RECOMMENDATIONS; TRASTUZUMAB;

D O I：

10.1002/smtd.201800122

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Blood-based detection and molecular phenotyping are highly desired for the early diagnosis and dynamic monitoring of cancer. Extracellular vesicles (EVs) carry molecular information from the cells of origin and are biomarkers of cancer. However, the detection and molecular analysis of EVs has been challenging due to their nanoscaled size. Here, an assessment of the detection and molecular phenotyping of serum EVs based on microbead-assisted flow cytometry is established. The clinical utility of this method is validated in the diagnosis and human epidermal growth factor receptor 2 (HER2) phenotyping of breast cancer. Good correlation between the status of epithelial cell adhesion molecule (EpCAM) and HER2 expression in EVs and in the cells of origin is found. Both EpCAM+ and HER2+ EVs are demonstrated to be effective diagnostic markers of breast cancer with high sensitivity and specificity. EV-based HER2 phenotyping is consistent with tissue-based HER2 phenotyping by immunohistochemistry and can be used as a surrogate for the invasive tissue assessments. The microbead-assisted flow cytometry assessment of EVs enables rapid and noninvasive detection and molecular phenotyping of cancer and would help to personalized treatment and cancer survival.

引用

页数：11

共 70 条

[1] Quantitative analysis of changes in ER, PR and HER2 expression in primary breast cancer and paired nodal metastases [J].