Antibacterial Activity of Silver Nanoparticles and Cinnamon Bark Extract Prepared by Pulse Laser Ablation and Plasma Jet Methods

This study explores a new facile method of obtaining plant extract using a atmospheric pressure and a way of producing Ag nanoparticles (Ag NPs) using the pulsed laser ablation(PLA) in distilled water. A 532 nm Nd:YAG laser at varying energies (500, 700, and 900 mJ) was used to prepare Ag NPs. Cinnamon bark extract was prepared by using an atmospheric pressure plasma jet in cinnamon bark and distilled water mixture with exposure times, (5 and 10 min). The physical characterization of these silver nanoparticles and Cinnamon bark extract were verified using X-ray diffraction patterns (XRD) , Field Emission Scanning Electron Microscopy(FESEM) and Ultraviolet-visible (UV-Vis) spectroscopy. The antibacterial activity of Ag NPs and a mixture (Ag NPs with Cinnamon extract) were evaluated for NPs obtained by PLA using different laser energy in distilled water, on the Gram-negative isolate (Escherichia coli). The results and images of inhibition zone diameter showed that the NPs have synergistic effects on the studied bacteria it increase with the increases of laser energy. A significant improvement in the bacterial inhibition of silver nanoparticles after mixing them with cinnamon bark extract.