Evaluation of NLRP3 (rs10754558) and PTPN22 (1858C/T) (rs2476601) Functional Polymorphisms in Psoriasis Susceptibility in Egypt

被引：7
作者：
ALrefai, Abeer [1 ,2 ]
Dawood, Ashraf [1 ]
Shehata, Wafaa [3 ]
Elhelbawy, Mohammed [4 ]
Elhelbawy, Nesreen [1 ]
机构：
[1] Menoufia Univ, Fac Med, Med Biochem & Mol Biol Dept, Shebin Elkom City, Egypt
[2] Umm Al Qura Univ, Fac Med, Biochem Dept, Makah City, Saudi Arabia
[3] Menoufia Univ, Fac Med, Dermatol Androl & Sexually Transmitted Dis STDs, Shebin Elkom City, Egypt
[4] Menoufia Univ, Fac Med, Clin Pathol Dept, Shebin Elkom City, Egypt
来源：
APPLICATION OF CLINICAL GENETICS | 2021年 / 14卷
关键词：
psoriasis; NLRP3 (rs10754558); PTPN22 (1858C/T) (rs2476601); real time PCR; INFLAMMASOME ACTIVATION; TYROSINE-PHOSPHATASE; AUTOIMMUNE-DISEASES; GENE POLYMORPHISMS; ASSOCIATION; ARTHRITIS; REVEALS; RISK;
D O I：
10.2147/TACG.S319065
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Background: Psoriasis is a complex autoimmune multifactorial disease induced by interaction of environmental and genetic factors. This research aimed to clarify the association of NLRP3 (rs10754558) and PTPN22 (1858C/T) (rs2476601) polymorphisms with susceptibility to psoriasis. Methods: This case-control study involved 150 patients diagnosed with psoriasis and 100 age-and gender-matched apparently healthy individuals. NLRP3 (rs10754558) polymorphism was done by real time PCR and PTPN22 1858C/T (rs2476601) genotype was identified by tetra-primer amplification refractory mutation system-polymerase chain reaction (PCR) method. Results: The genotypes distribution of NLRP3 (rs10754558) were significantly associated with psoriasis (p<0.0001). Whereas for PTPN22 (1858C/T) (rs2476601), no significance was found (p=0.09). NLRP3 (rs10754558) GC genotype revealed a significant association with psoriasis (p<0.0001), mainly among male (p=0.004) patients with mild psoriasis (p=0.001) and affected extremities (p=0.0001). Conclusion: We can conclude that the NLRP3 (rs10754558) GC genotype may play a role in psoriasis susceptibility among male Egyptian populations with affected extremities. Future studies must evaluate its role in the prevention or the treatment of psoriasis.
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页码：331 / 339
页数：9
相关论文
共 37 条
[1]   Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder [J].
Afonina, I ;
Zivarts, M ;
Kutyavin, I ;
Lukhtanov, E ;
Gamper, H ;
Meyer, RB .
NUCLEIC ACIDS RESEARCH, 1997, 25 (13) :2657-2660
[2]  
Al-Awadhi A, 2020, OPEN RHEUMATOL J, V14, P15, DOI [10.2174/1874312902014010015, DOI 10.2174/1874312902014010015]
[3]   PTPN22 Polymorphism is Related to Autoimmune Disease Risk in Patients with Turner Syndrome [J].
Bianco, B. ;
Verreschi, I. T. N. ;
Oliveira, K. C. ;
Guedes, A. D. ;
Galera, B. B. ;
Galera, M. F. ;
Barbosa, C. P. ;
Lipay, M. V. N. .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2010, 72 (03) :256-259
[4]  
Bin Huraib G, 2017, CLIN MED INSIGHTS AR, V11, P1
[5]   Inhibition of inflammasome activation improves the impaired pattern of healing in genetically diabetic mice [J].
Bitto, Alessandra ;
Altavilla, Domenica ;
Pizzino, Gabriele ;
Irrera, Natasha ;
Pallio, Giovanni ;
Colonna, Michele R. ;
Squadrito, Francesco .
BRITISH JOURNAL OF PHARMACOLOGY, 2014, 171 (09) :2300-2307
[6]   Psoriasis [J].
Boehncke, Wolf-Henning ;
Schoen, Michael P. .
LANCET, 2015, 386 (9997) :983-994
[7]   PTPN22 is associated with susceptibility to psoriatic arthritis but not psoriasis: evidence for a further PsA-specific risk locus [J].
Bowes, John ;
Loehr, Sabine ;
Budu-Aggrey, Ashley ;
Uebe, Steffen ;
Bruce, Ian N. ;
Feletar, Marie ;
Marzo-Ortega, Helena ;
Helliwell, Philip ;
Ryan, Anthony W. ;
Kane, David ;
Korendowych, Eleanor ;
Alenius, Gerd-Marie ;
Giardina, Emiliano ;
Packham, Jonathan ;
McManus, Ross ;
FitzGerald, Oliver ;
Brown, Matthew A. ;
Behrens, Frank ;
Burkhardt, Harald ;
McHugh, Neil ;
Huffmeier, Ulrike ;
Ho, Pauline ;
Reis, Andre ;
Barton, Anne .
ANNALS OF THE RHEUMATIC DISEASES, 2015, 74 (10) :1882-1885
[8]  
Chandy Sharon, 2020, Our Dermatology Online, V11, P275, DOI 10.7241/ourd.20204.13
[9]   PTPN22 C1858T and the risk of psoriasis: a meta-analysis [J].
Chen, Yu-Fu ;
Chang, Jeffrey S. .
MOLECULAR BIOLOGY REPORTS, 2012, 39 (08) :7861-7870
[10]   Targeting the NLRP3 Inflammasome to Reduce Diet-Induced Metabolic Abnormalities in Mice [J].
Chiazza, Fausto ;
Couturier-Maillard, Aurelie ;
Benetti, Elisa ;
Mastrocola, Raffaella ;
Nigro, Debora ;
Cutrin, Juan C. ;
Serpe, Loredana ;
Aragno, Manuela ;
Fantozzi, Roberto ;
Ryffel, Bernard ;
Thiemermann, Christoph ;
Collino, Massimo .
MOLECULAR MEDICINE, 2015, 21 :1025-1037
1234