Assessing the functionality of a membrane protein in a three-dimensional crystal

被引：47
作者：
Heberle, J
Büldt, G
Koglin, E
Rosenbusch, JP
Landau, EM
机构：
[1] Forschungszentrum Julich, Inst Biol & Informationsverarbeitung, Struct Biol, D-52425 Julich, Germany
[2] Forschungszentrum Julich, Inst Chem & Dynam Geosphare, D-52425 Julich, Germany
[3] Univ Basel, Biozentrum, CH-4056 Basel, Switzerland
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1998年 / 281卷 / 04期
关键词：
FT-IR; time-resolved; proton transfer; bacteriorhodopsin; lipidic cubic phase crystallization;
D O I：
10.1006/jmbi.1998.1970
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hexagonal microcrystals of bacteriorhodopsin embedded in a lipidic cubic phase have been investigated by time-resolved FT-IR and resonance Raman spectroscopy. Retinal isomerization, conformational changes in the protein backbone and proton translocation are virtually indistinguishable from those in the native membrane. The protein is thus fully active in three-dimensional crystals. (C) 1998 Academic Press.
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收藏
页码：587 / 592
页数：6
相关论文
共 24 条
[1]  
Althaus T, 1995, ISRAEL J CHEM, V35, P227
[2]  
DANSHINA SV, 1992, PHOTOCHEM PHOTOBIOL, V55, P735
[3]   STRUCTURAL-CHANGES IN BACTERIORHODOPSIN DURING PROTON TRANSLOCATION REVEALED BY NEUTRON-DIFFRACTION [J].
DENCHER, NA ;
DRESSELHAUS, D ;
ZACCAI, G ;
BULDT, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (20) :7876-7879
[4]   FTIR STUDIES ON CRYSTALS OF PHOTOSYNTHETIC REACTION CENTERS [J].
GERWERT, K ;
HESS, B ;
MICHEL, H ;
BUCHANAN, S .
FEBS LETTERS, 1988, 232 (02) :303-307
[5]   Electron-crystallographic refinement of the structure of bacteriorhodopsin [J].
Grigorieff, N ;
Ceska, TA ;
Downing, KH ;
Baldwin, JM ;
Henderson, R .
JOURNAL OF MOLECULAR BIOLOGY, 1996, 259 (03) :393-421
[6]   General concept fur ion translocation by halobacterial retinal proteins: The isomerization/switch/transfer (IST) model [J].
Haupts, U ;
Tittor, J ;
Bamberg, E ;
Oesterhelt, D .
BIOCHEMISTRY, 1997, 36 (01) :2-7
[7]   ATR/FT-IR difference spectroscopy of biological matter with microsecond time resolution [J].
Heberle, J ;
Zscherp, C .
APPLIED SPECTROSCOPY, 1996, 50 (05) :588-596
[8]   MODEL FOR THE STRUCTURE OF BACTERIORHODOPSIN BASED ON HIGH-RESOLUTION ELECTRON CRYOMICROSCOPY [J].
HENDERSON, R ;
BALDWIN, JM ;
CESKA, TA ;
ZEMLIN, F ;
BECKMANN, E ;
DOWNING, KH .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 213 (04) :899-929
[9]   3-DIMENSIONAL MODEL OF PURPLE MEMBRANE OBTAINED BY ELECTRON-MICROSCOPY [J].
HENDERSON, R ;
UNWIN, PNT .
NATURE, 1975, 257 (5521) :28-32
[10]   Surface of bacteriorhodopsin revealed by high-resolution electron crystallography [J].
Kimura, Y ;
Vassylyev, DG ;
Miyazawa, A ;
Kidera, A ;
Matsushima, M ;
Mitsuoka, K ;
Murata, K ;
Hirai, T ;
Fujiyoshi, Y .
NATURE, 1997, 389 (6647) :206-211
123