Purification and characterisation of an ester hydrolase from a strain of Arthrobacter species:: Its application in asymmetrisation of 2-benzyl-1,3-propanediol acylates

被引:37
作者
Johri, S [1 ]
Verma, V [1 ]
Parshad, R [1 ]
Koul, S [1 ]
Taneja, SC [1 ]
Qazi, GN [1 ]
机构
[1] Reg Res Lab, CSIR, Jammu 180001, India
关键词
D O I
10.1016/S0968-0896(00)00240-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An ester hydrolase (ABL) has been isolated from a strain of Arthrobacter species (RRLJ-1/95) maintained in the culture collection of this laboratory. The purified enzyme has a specific activity of 1700 U/mg protein and is found to be composed of a single subunit (Mr 32,000), exhibiting both lipase and esterase activities shown by hydrolysis of triglycerides and p-nitrophenyl acetate respectively. Potential application of the enzyme concerns the asymmetrisation of prochiral 2-benzyl-1,3-propanediol esters besides enantioselective hydrolysis of alkyl esters of unsubstituted and substituted 1-phenyl ethanols. (C) 2001 Elsevier Science Ltd. All rights reserved.
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页码:269 / 273
页数:5
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