Urine-based regenerative RNA biomarkers for urinary bladder wound healing

被引：1
作者：
Liu, Chanzhen [1 ,2 ]
Zhou, Juan [1 ,2 ]
Li, You [3 ]
Lu, Yulei [1 ,2 ]
Lu, Haoyuan [1 ,2 ]
Wei, Wei [1 ,2 ]
Wu, Mingsong [4 ,5 ]
Yi, Xianlin [1 ,2 ]
机构：
[1] GuangxiMed Univ, Canc Hosp, Nanning 530021, Peoples R China
[2] Guangxi Canc Res Inst, Nanning 530021, Peoples R China
[3] East China Normal Univ, Life Sci Inst, Shanghai 200241, Peoples R China
[4] Zunyi Med Univ, Sch Stomatol, Zunyi 563006, Guizhou, Peoples R China
[5] Zunyi Med Univ, Special Key Lab Oral Dis Res Higher Educ Inst Gui, Zunyi 563000, Guizhou, Peoples R China
来源：
REGENERATIVE MEDICINE | 2021年 / 16卷 / 08期
关键词：
bladder injury; cholinergic receptor; regeneration; stem cell; urine; STEM-CELL; UROTHELIUM; HYPOXIA;
D O I：
10.2217/rme-2021-0057
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Background: This study aimed to investigate the expression of regeneration-related genes in canine urine during bladder repair. Materials & methods: Canine urine samples were collected after partial cystectomy. Regenerative mRNA of hypoxia-inducible factor (HIF), vascular endothelial growth factor (VEGF), key stem cell transcription factors and cholinergic signals were detected. Results: HIF-1 alpha, VEGF, CD44, IL-6 and prominin-1 expression in canine urine after partial cystectomy exhibited two similar peaks at similar to 2 weeks. HIF-1 alpha and VEGF expression were higher in the afternoon than the morning. The expression of key stem cell transcription factors and cholinergic signals also exhibited a rhythm along with bladder healing. Conclusions: The expression of HIF-1 alpha, VEGF, key stem cell transcription factors and cholinergic signals exhibited a time curve distribution during canine bladder healing. The expression trend of some regenerative genes was similar during bladder healing, and a cooperative effect may exist.
引用
收藏
页码：709 / 718
页数：10
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