Dissemination and persistence of extended-spectrum cephalosporin-resistance encoding IncI1-blaCTXM-1 plasmid among Escherichia coli in pigs

被引：56

作者：

Abraham, Sam ^{[1
]}

Kirkwood, Roy N. ^{[2
,3
]}

Laird, Tanya ^{[1
]}

Saputra, Sugiyono ^{[2
,3
,4
]}

Mitchell, Tahlia ^{[2
,3
]}

Singh, Mohinder ^{[2
,3
]}

Linn, Benjamin ^{[2
,3
]}

Abraham, Rebecca J. ^{[3
]}

Pang, Stanley ^{[1
]}

Gordon, David M. ^{[5
]}

Trott, Darren J. ^{[2
,3
]}

O'Dea, Mark ^{[1
]}

机构：

[1] Murdoch Univ, Sch Vet & Life Sci, Antimicrobial Resistance & Infect Dis Lab, Melbourne, WA, Australia

[2] Univ Adelaide, Sch Anim & Vet Sci, Roseworthy, Australia

[3] Univ Adelaide, Australian Ctr Antimicrobial Resistance Ecol, Adelaide, SA, Australia

[4] Indonesian Inst Sci, Res Ctr Biol, Cibinong, West Java, Indonesia

[5] Australian Natl Univ, Res Sch Biol, Evolut Ecol & Genet, Canberra, ACT, Australia

来源：

ISME JOURNAL | 2018年 / 12卷 / 10期

关键词：

FOOD-PRODUCING ANIMALS; ANTIMICROBIAL RESISTANCE; PUBLIC-HEALTH; TRANSMISSION; ASSOCIATION; IDENTIFICATION; LIVESTOCK; CARRIAGE; DYNAMICS; POULTRY;

D O I：

10.1038/s41396-018-0200-3

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

This study investigated the ecology, epidemiology and plasmid characteristics of extended-spectrum cephalosporin (ESC)-resistant E. coli in healthy pigs over a period of 4 years (2013-2016) following the withdrawal of ESCs. High carriage rates of ESC-resistant E. coli were demonstrated in 2013 (86.6%) and 2014 (83.3%), compared to 2015 (22%) and 2016 (8.5%). ESC resistance identified among E. coli isolates was attributed to the carriage of an IncI1 ST-3 plasmid (pCTXM1-MU2) encoding bla(CTXM)(-)(1). Genomic characterisation of selected E. coli isolates (n = 61) identified plasmid movement into multiple commensal E. coli (n = 22 STs). Major STs included ST10, ST5440, ST453, ST2514 and ST23. A subset of the isolates belong to the atypical enteropathogenic E. coli (aEPEC) pathotype that harboured multiple LEE pathogenic islands. pCTXM1-MU2 was similar (99% nt identity) to IncI1-ST3 plasmids reported from Europe, encoded resistance to aminoglycosides, sulphonamides and trimethoprim, and carried colicin Ib. pCTXM1-MU2 appears to be highly stable and readily transferable. This study demonstrates that ESC resistance may persist for a protracted period following removal of direct selection pressure, resulting in the emergence of ESC-resistance in both commensal E. coli and aEPEC isolates of potential significance to human and animal health.

引用

页码：2352 / 2362

页数：11

共 51 条

[1] First detection of extended-spectrum cephalosporin- and fluoroquinolone-resistant Escherichia coli in Australian food-producing animals [J].