Enhanced Immunosensor Using a Handheld pH Meter for the Point-of-care, Sensitive Detection of Prostate Specific Antigen

被引：7

作者：

Gao, Rong ^{[1
]}

Liu, Bingqian ^{[1
]}

Luo, Dajuan ^{[1
]}

Su, Yonghuan ^{[1
]}

Su, Lixia ^{[1
]}

机构：

[1] Guizhou Univ, Coll Pharm, Engn Lab Synthet Drugs, Minist Educ Guizhou Prov, Guiyang 550025, Guizhou, Peoples R China

来源：

ELECTROANALYSIS | 2022年 / 34卷 / 02期

基金：

中国国家自然科学基金;

关键词：

point-of-care; handheld pH meter; Polyamide-amine dendrite; Prostate specific antigen; HYBRIDIZATION CHAIN-REACTION; SIGNAL AMPLIFICATION; GLUCOSE METER; IMMUNOASSAY; CANCER; MARKERS;

D O I：

10.1002/elan.202100285

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enhanced handheld pH meter based immunosensor was proposed for point-of-care, quantitative determination of prostate specific antigen (PSA). Monoclonal antibody-functionalized magnetic beads (MB-Ab(1)) were prepared to capture PSA in sample, and subsequently bond to polyclonal antibody-immobilized gold nanoparticles-polyamide-amine dendrite-lead ion (GO-PAMAM-Pb2+) and dispersed in H2S solution. Based on negative Gibbs free energy of formation, PbS (solubility product constant K-sp=8.0x10(-28) was formed by reaction of Pb2+ and H2S. Hence, the pH of solution increased due to the H+ appeared, and the resulting pH value was monitored by a handheld pH meter.

引用

页码：275 / 280

页数：6

共 45 条

[1] Ionic liquid-decorated Fe3O4@SiO2 nanocomposite coated on talc sheets: An efficient adsorbent for methylene blue in aqueous solution [J].

Alizadeh, A. ;

Fakhari, M. ;

Safaei, Z. ;

Khodeai, M. M. ;

Repo, E. ;

Asadi, A. .

INORGANIC CHEMISTRY COMMUNICATIONS, 2020, 121

[2]

Centers for Disease Control and Prevention, 2019, MMWR MORB MORTAL WKL, V68, P531

[3] A label-free electrochemical immunosensor based on facet-controlled Au nanorods/reduced graphene oxide composites for prostate specific antigen detection [J].

Chen, Shuzhe ;

Xu, Lin ;

Sheng, Kuang ;

Zhou, Qingqing ;

Dong, Biao ;

Bai, Xue ;

Lu, Geyu ;

Song, Hongwei .

SENSORS AND ACTUATORS B-CHEMICAL, 2021, 336

[4]

De Angelis Gabriela, 2007, Rev Urol, V9, P113

[5]

Dean J.A., 1985, LANGES HDB CHEM, V13

[6] Multiplexed tri-mode visual outputs of immunoassay signals on a clip-magazine-assembled photothermal biosensing disk [J].

Fu, Guanglei ;

Li, Xiujun ;

Wang, Weihua ;

Hou, Ruixia .

BIOSENSORS & BIOELECTRONICS, 2020, 170 (170)

[7] Synthesis of a morphology controllable Fe3O4 nanoparticle/hydrogel magnetic nanocomposite inspired by magnetotactic bacteria and its application in H2O2 detection [J].

Gao, Yang ;

Wei, Zhao ;

Li, Fei ;

Yang, Zhi Mao ;

Chen, Yong Mei ;

Zrinyi, Miklos ;

Osada, Yoshihito .

GREEN CHEMISTRY, 2014, 16 (03) :1255-1261

[8] PSA markers in prostate cancer detection [J].

Gretzer, MB ;

Partin, AW .

UROLOGIC CLINICS OF NORTH AMERICA, 2003, 30 (04) :677-+

[9] Integrating Near-Infrared Visual Fluorescence with a Photoelectrochemical Sensing System for Dual Readout Detection of Biomolecules [J].

Han, Qingzhi ;

Zhao, Xuan ;

Na, Na ;

Ouyang, Jin .

ANALYTICAL CHEMISTRY, 2021, 93 (07) :3486-3492

[10] A supersandwich electrochemiluminescence immunosensor based on mimic-intramolecular interaction for sensitive detection of proteins [J].

He, Ying ;

Chai, Yaqin ;

Yuan, Ruo ;

Wang, Haijun ;

Bai, Lijuan ;

Liao, Ni .

ANALYST, 2014, 139 (20) :5209-5214

← 1 2 3 4 5 →