Preparation of sustained-release gelatin microspheres as the cell microcarrier

The BSA-impregnated large diameter gelatin microspheres were prepared, with the improved and emulsified cold-condensation method. The results indicate that the gelatin solution 25% ( mass fraction) volume ratio(3: 20) of water phase to oil phase, stirring rate 300 r/min, glutaraldehyde 0.1 mL(25%, mass fraction), and surfactant span-80 0.1 g were an optimal condition to prepare the gelatin microsphere. The average diameter of microspheres was 250 mu m. By using three different ways to process the microspheres the gelatin microspheres with the different surface structures and cell adsorption ratio could be obtained. The blank microspheres can biodegrade absolutely in vitro. The BSA release from the BSA-impregnated gelatin microsphere can be sustained for 30 d. Fibroblasts grew well on the surface of gelatin microcarrier.