Characterization of Magnetic Markers for Liquid-Phase Immunoassays Using Brownian Relaxation

被引：20

作者：

Enpuku, Keiji ^{[1
]}

Watanabe, Hideki ^{[1
]}

Higuchi, Yuichi ^{[1
]}

Yoshida, Takashi ^{[1
]}

Kuma, Hiroyuki ^{[2
]}

Hamasaki, Naotaka ^{[2
]}

Mitsunaga, Masakazu ^{[3
]}

Kanzaki, Hisao ^{[3
]}

Kandori, Akihiko ^{[4
]}

机构：

[1] Kyushu Univ, Res Inst Superconductor Sci & Syst, Fukuoka 8190395, Japan

[2] Nagasaki Int Univ, Fac Pharmaceut Sci, Sasebo, Nagasaki 8593298, Japan

[3] Hitachi Maxell Ltd, R&D Div, Kyoto 6188525, Japan

[4] Hitachi Ltd, Cent Res Lab, Kokubunji, Tokyo 1858601, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS | 2012年 / 51卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

SIZE DISTRIBUTION; SUSCEPTIBILITY; SENSOR;

D O I：

10.1143/JJAP.51.023002

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We characterized the magnetic markers used in biological immunoassays based on Brownian relaxation. Because the markers are composed of aggregated nanoparticles, i.e., magnetic nanoclusters, we first clarified their magnetic properties using AC susceptibility measurements, magnetization (M-H) curves, and magnetic relaxation properties. Analyzing the experimental results, we obtained the key parameters for the immunoassay, i.e., hydrodynamic diameter d(h), magnetic moment m(B), and anisotropy energy E-B of the markers. Because these parameters were distributed in practical samples, we took their distribution into account in the analysis. Next, we showed the relationship between these parameters obtained from different samples. It was shown that m(B) increased approximately in proportion to d(h). On the other hand, no clear correlation between m(B) and E-B was obtained. These results were very different from those expected from single-domain nanoparticles and must be taken into account when magnetic markers are used in immunoassays based on Brownian relaxation. (C) 2012 The Japan Society of Applied Physics

引用

页数：7

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