Effect of -glucan on MUC4 and MUC5B expression in human airway epithelial cells

Background-Glucan is found in the cell walls of fungi, bacteria, and some plant tissues, and is detected by the innate immune system. Furthermore, this recognition is known to worsen respiratory symptoms in patients with allergic and inflammatory airway diseases. However, the means by which -glucan affects the secretion of major mucins by human airway epithelial cells has not been elucidated. Therefore, in this study, the effect and signaling pathway of -glucan on mucins MUC4 and MUC5B were investigated in human airway epithelial cells. MethodsIn NCI-H292 cells and human normal nasal epithelial cells, the effect and signaling pathway of -glucan on MUC4 and MUC5B expression were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR, enzyme immunoassay, and immunoblot analysis with specific inhibitors and small interfering RNA (siRNA). Results-Glucan increased MUC4 and MUC5B expression and activated the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B). SB203580 (a p38 MAPK inhibitor) and pyrrolidine dithiocarbamate (PDTC; a NF-B inhibitor) inhibited -glucan-induced MUC4 and MUC5B expression. In addition, siRNA knockdown of p38 MAPK blocked -glucan-induced MUC4 and MUC5B mRNA expression and -glucan-activated phosphorylation of NF-B. Furthermore, Toll-like receptor 4 (TLR4) mRNA expression was increased by -glucan, and siRNA knockdown of TLR4 blocked -glucan-induced MUC4 and MUC5B mRNA expression and -glucan-activated phosphorylation of p38 MAPK and NF-B. ConclusionThese results demonstrate that in human airway epithelial cells -glucan induces MUC4 and MUC5B expression via the TLR4-p38 MAPK-NF-B signaling pathway.