Design and application of 2-D DGGE-based gene mutational scanning tests

被引：9

作者：

Van Orsouw, NJ ^{[1
]}

Vijg, J ^{[1
]}

机构：

[1] Canc Therapy & Res Ctr S Texas, Inst Drug Dev, Basic Biol Sect, San Antonio, TX 78229 USA

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1999年 / 14卷 / 5-6期

关键词：

2-D DGGE; gene mutational scanning tests; PCR;

D O I：

10.1016/S1050-3862(98)00028-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Currently, there is a need for practical, accurate and cost-efficient tests to comprehensively scan human genes for disease-related DNA sequence variation. Two-dimensional gene scanning (TDGS) is a parallel mutation detection system, based on a combination of extensive multiplex PCR amplification ('PCR megaplex') and two-dimensional (2-D) DNA electrophoresis. The latter comprises a size separation step followed by denaturing gradient gel electrophoresis (DGGE), and allows single base pair changes to be distinguished among multiple DNA fragments in parallel. Here, we describe the rapid design of TDGS tests and its application to mutation identification in several large human cancer genes. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：205 / 213

页数：9

共 26 条

[11]

Foulkes WD, 1995, CLIN INVEST MED, V18, P473

[12] MOLECULAR ANALYSIS OF PHENYLKETONURIA IN DENMARK - 99-PERCENT OF THE MUTATIONS DETECTED BY DENATURING GRADIENT GEL-ELECTROPHORESIS [J].

GULDBERG, P ;

HENRIKSEN, KF ;

GUTTLER, F .

GENOMICS, 1993, 17 (01) :141-146

[13] Multiplex co-amplification of 24 retinoblastoma gene exons after pre-amplification by long-distance PCR [J].

Li, DZ ;

Vijg, J .

NUCLEIC ACIDS RESEARCH, 1996, 24 (03) :538-539

[14] GERM LINE P53 MUTATIONS IN A FAMILIAL SYNDROME OF BREAST-CANCER, SARCOMAS, AND OTHER NEOPLASMS [J].

MALKIN, D ;

LI, FP ;

STRONG, LC ;

FRAUMENI, JF ;

NELSON, CE ;

KIM, DH ;

KASSEL, J ;

GRYKA, MA ;

BISCHOFF, FZ ;

TAINSKY, MA ;

FRIEND, SH .

SCIENCE, 1990, 250 (4985) :1233-1238

[15]

MOYRET C, 1994, ONCOGENE, V9, P1739

[16] PARALLEL GENOME ANALYSIS BY 2-DIMENSIONAL DNA TYPING [J].

MULLAART, E ;

DEVOS, GJ ;

MEERMAN, GJT ;

UITTERLINDEN, AG ;

VIJG, J .

NATURE, 1993, 365 (6445) :469-471

[17]

MYERS RM, 1985, NUCLEIC ACIDS RES, V13, P3131, DOI 10.1093/nar/13.9.3131

[18]

MYERS RM, 1987, METHOD ENZYMOL, V155, P501

[19] Comprehensive mutational scanning of the p53 coding region by two-dimensional gene scanning [J].

Rines, RD ;

van Orsouw, NJ ;

Sigalas, I ;

Li, FP ;

Eng, C ;

Vijg, J .

CARCINOGENESIS, 1998, 19 (06) :979-984

[20]

Sasaki S, 1997, HUM MUTAT, V9, P164

← 1 2 3 →