Design and application of 2-D DGGE-based gene mutational scanning tests

被引：9

作者：

Van Orsouw, NJ ^{[1
]}

Vijg, J ^{[1
]}

机构：

[1] Canc Therapy & Res Ctr S Texas, Inst Drug Dev, Basic Biol Sect, San Antonio, TX 78229 USA

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1999年 / 14卷 / 5-6期

关键词：

2-D DGGE; gene mutational scanning tests; PCR;

D O I：

10.1016/S1050-3862(98)00028-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Currently, there is a need for practical, accurate and cost-efficient tests to comprehensively scan human genes for disease-related DNA sequence variation. Two-dimensional gene scanning (TDGS) is a parallel mutation detection system, based on a combination of extensive multiplex PCR amplification ('PCR megaplex') and two-dimensional (2-D) DNA electrophoresis. The latter comprises a size separation step followed by denaturing gradient gel electrophoresis (DGGE), and allows single base pair changes to be distinguished among multiple DNA fragments in parallel. Here, we describe the rapid design of TDGS tests and its application to mutation identification in several large human cancer genes. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：205 / 213

页数：9

共 26 条

[1] COMPREHENSIVE DETECTION OF SINGLE BASE CHANGES IN HUMAN GENOMIC DNA USING DENATURING GRADIENT GEL-ELECTROPHORESIS AND A GC CLAMP [J].

ABRAMS, ES ;

MURDAUGH, SE ;

LERMAN, LS .

GENOMICS, 1990, 7 (04) :463-475

[2] COMPLETE SEQUENCING OF THE P53 GENE PROVIDES PROGNOSTIC INFORMATION IN BREAST-CANCER PATIENTS, PARTICULARLY IN RELATION TO ADJUVANT SYSTEMIC THERAPY AND RADIOTHERAPY [J].

BERGH, J ;

NORBERG, T ;

SJOGREN, S ;

LINDGREN, A ;

HOLMBERG, L .

NATURE MEDICINE, 1995, 1 (10) :1029-1034

[3] Critical factors in the performance and cost of two-dimensional gene scanning: RB1 as a model [J].

Dhanda, RK ;

van Orsouw, NJ ;

Sigalas, I ;

Eng, C ;

Vijg, J .

BIOTECHNIQUES, 1998, 25 (04) :664-+

[4]

DHANDA RK, 1998, J GENET TESTING, V19, P979

[5]

EDWARDS MC, 1994, PCR METH APPL, V3, pS65

[6]

Eng C, 1997, CANCER EPIDEM BIOMAR, V6, P379

[7] Genetic testing: The problems and the promise [J].

Eng, C ;

Vijg, J .

NATURE BIOTECHNOLOGY, 1997, 15 (05) :422-426

[8] LENGTH-INDEPENDENT SEPARATION OF DNA RESTRICTION FRAGMENTS IN 2-DIMENSIONAL GEL-ELECTROPHORESIS [J].

FISCHER, SG ;

LERMAN, LS .

CELL, 1979, 16 (01) :191-200

[9] DNA FRAGMENTS DIFFERING BY SINGLE BASE-PAIR SUBSTITUTIONS ARE SEPARATED IN DENATURING GRADIENT GELS - CORRESPONDENCE WITH MELTING THEORY [J].

FISCHER, SG ;

LERMAN, LS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (06) :1579-1583

[10] RISKS OF CANCER IN BRCA1-MUTATION CARRIERS [J].

FORD, D ;

EASTON, DF ;

BISHOP, DT ;

NAROD, SA ;

GOLDGAR, DE ;

HAITES, N ;

MILNER, B ;

ALLAN, L ;

PONDER, BAJ ;

PETO, J ;

SMITH, S ;

STRATTON, M ;

LENOIR, GM ;

FEUNTEUN, J ;

LYNCH, H ;

ARASON, A ;

BARKARDOTTIR, R ;

EGILSSON, V ;

BLACK, DM ;

KELSELL, D ;

SPURR, N ;

DEVILEE, P ;

CORNELISSE, CJ ;

VARSEN, H ;

BIRCH, JM ;

SKOLNICK, M ;

SANTIBANEZKOREF, MS ;

TEARE, D ;

STEEL, M ;

PORTER, D ;

COHEN, BB ;

CAROTHERS, A ;

SMYTH, E ;

WEBER, B ;

NEWBOLD, B ;

BOEHNKE, M ;

COLLINS, FS ;

CANNONALBRIGHT, LA ;

GOLDGAR, D .

LANCET, 1994, 343 (8899) :692-695

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