Chemical analysis of multicellular tumour spheroids

被引:44
作者
Jamieson, L. E. [1 ]
Harrison, D. J. [2 ]
Campbell, C. J. [1 ]
机构
[1] Univ Edinburgh, Sch Chem, EaStCHEM, Edinburgh EH9 3JJ, Midlothian, Scotland
[2] Univ St Andrews, St Andrews KY16 9TF, Fife, Scotland
关键词
LIGHT-SHEET MICROSCOPY; PROTEIN EXPRESSION; HYPOXIC CELLS; IN-VITRO; EXTRACELLULAR-MATRIX; MASS-SPECTROMETRY; LIVE CELLS; CULTURE; PH; GROWTH;
D O I
10.1039/c5an00524h
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Conventional two dimensional (2D) monolayer cell culture has been considered the 'gold standard' technique for in vitro cellular experiments. However, the need for a model that better mimics the three dimensional (3D) architecture of tissue in vivo has led to the development of Multicellular Tumour Spheroids (MTS) as a 3D tissue culture model. To some extent MTS mimic the environment of in vivo tumours where, for example, oxygen and nutrient gradients develop, protein expression changes and cells form a spherical structure with regions of proliferation, senescence and necrosis. This review focuses on the development of techniques for chemical analysis of MTS as a tool for understanding in vivo tumours and a platform for more effective drug and therapy discovery. While traditional monolayer techniques can be translated to 3D models, these often fail to provide the desired spatial resolution and z-penetration for live cell imaging. More recently developed techniques for overcoming these problems will be discussed with particular reference to advances in instrument technology for achieving the increased spatial resolution and imaging depth required.
引用
收藏
页码:3910 / 3920
页数:11
相关论文
共 84 条
[1]   Correlated mass spectrometry imaging and confocal Raman microscopy for studies of three-dimensional cell culture sections [J].
Ahlf, Dorothy R. ;
Masyuko, Rachel N. ;
Hummon, Amanda B. ;
Bohn, Paul W. .
ANALYST, 2014, 139 (18) :4578-4585
[2]   Microscopic images of intraspheroidal pH by 1H magnetic resonance chemical shift imaging of pH sensitive indicators [J].
Alvarez-Pérez, J ;
Ballesteros, P ;
Cerdán, S .
MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE, 2005, 18 (06) :293-301
[3]   Phenotypic Profiling of Raf Inhibitors and Mitochondrial Toxicity in 3D Tissue Using Biodynamic Imaging [J].
An, Ran ;
Merrill, Dan ;
Avramova, Larisa ;
Sturgis, Jennifer ;
Tsiper, Maria ;
Robinson, J. Paul ;
Turek, John ;
Nolte, David D. .
JOURNAL OF BIOMOLECULAR SCREENING, 2014, 19 (04) :526-537
[4]   An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids [J].
Anada, Takahisa ;
Fukuda, Junji ;
Sai, Yuko ;
Suzuki, Osamu .
BIOMATERIALS, 2012, 33 (33) :8430-8441
[5]   Using Fluorescence Lifetime Imaging Microscopy to Monitor Theranostic Nanoparticle Uptake and Intracellular Doxorubicin Release [J].
Basuki, Johan S. ;
Duong, Hien T. T. ;
Macmillan, Alexander ;
Erlich, Rafael B. ;
Esser, Lars ;
Akerfeldt, Mia C. ;
Whan, Renee Megan ;
Kavallaris, Maria ;
Boyer, Cyrille ;
Davis, Thomas P. .
ACS NANO, 2013, 7 (11) :10175-10189
[6]   All-optical nanoscale pH meter [J].
Bishnoi, Sandra W. ;
Rozell, Christopher J. ;
Levin, Carly S. ;
Gheith, Muhammed K. ;
Johnson, Bruce R. ;
Johnson, Don H. ;
Halas, Naomi J. .
NANO LETTERS, 2006, 6 (08) :1687-1692
[7]   BIOLOGICAL RESPONSE OF MULTICELLULAR EMT6 SPHEROIDS TO EXOGENOUS LACTATE [J].
BOURRATFLOECK, B ;
GROEBE, K ;
MUELLERKLIESER, W .
INTERNATIONAL JOURNAL OF CANCER, 1991, 47 (05) :792-799
[8]   Automated subcellular localization and quantification of protein expression in tissue microarrays [J].
Camp, RL ;
Chung, GG ;
Rimm, DL .
NATURE MEDICINE, 2002, 8 (11) :1323-1327
[9]   Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging [J].
Centonze, VE ;
White, JG .
BIOPHYSICAL JOURNAL, 1998, 75 (04) :2015-2024
[10]   A MARKER FOR HYPOXIC CELLS IN TUMORS WITH POTENTIAL CLINICAL APPLICABILITY [J].
CHAPMAN, JD ;
FRANKO, AJ ;
SHARPLIN, J .
BRITISH JOURNAL OF CANCER, 1981, 43 (04) :546-550