Alkannin/shikonin mixture from roots of Onosma echioides (L.) L.:: Extraction method study and quantification

被引：26

作者：

Sagratini, Gianni ^{[1
]}

Cristalli, Gloria ^{[1
]}

Giardina, Dario ^{[1
]}

Gioventu, Giorgio ^{[1
]}

Maggi, Filippo ^{[2
]}

Ricciutelli, Massimo ^{[1
]}

Vittori, Sauro ^{[1
]}

机构：

[1] Univ Camerino, Dept Chem Sci, Fac Pharm, I-62032 Camerino, Italy

[2] Univ Camerino, Dept Environm Sci, Fac Pharm, Sect Bot & Ecol, I-62032 Camerino, Italy

来源：

JOURNAL OF SEPARATION SCIENCE | 2008年 / 31卷 / 6-7期

关键词：

extraction; HPLC; naphthoquinones; Onosma;

D O I：

10.1002/jssc.200700408

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work reports the extraction procedures of alkannin/shikonin mixture from roots of six populations of Onosma echioides, by means of three extraction techniques: Soxhlet extraction, maceration and rapid solid-liquid dynamic extraction (RSLDE). Five solvents with different polarity (hexane, petroleum ether, chloroform, ethyl acetate, methanol) were also studied. Analysis of the extracts was performed by an HPLC-DAD (diode array detector) system. The most efficient extraction technique was Soxhlet procedure using ethyl acetate for 6 h. Studied samples of O. echioides showed an alkannin/shikonin content in the range of 0.02 - 0.24 mg/kg. Other naphthoquinone derivatives (deoxyalkannin/deoxyshikonin and 5,8-dihydroxy-2-(4-methyl-6-oxo-5,6-dihydro-2H-pyran-2-yl)-[1,4]naphthoquinone and arnebin-6) were found for the first time in O. echioides and characterized in the extracts using HPLC-MS apparatus equipped with an ESI ionization source.

引用

页码：945 / 952

页数：8

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