In vivo electrochemical impedance measurement on single cell membrane

被引：5

作者：

Bai, Seoung-Jai ^{[1
]}

Prinz, Fritz B. ^{[2
,3
]}

机构：

[1] Dankook Univ, Dept Mech Engn, Yongin 448701, South Korea

[2] Stanford Univ, Dept Mech Engn, Nanoscale Prototyping Lab Energy Convers & Storag, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA

来源：

MICROELECTRONIC ENGINEERING | 2011年 / 88卷 / 10期

关键词：

Electrochemical impedance spectroscopy (EIS); Cell plasma membrane; Single cell measurement; Ultra-microelectrode (UME); ELECTRICAL-IMPEDANCE;

D O I：

10.1016/j.mee.2011.06.003

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

The electrochemical characteristics of the plasma membranes of single cells and organelles were investigated. Silicon fabrication technology was used to produce a metal ultra-microelectrode (UME). Furthermore, the UME was characterized in a cell medium using electrochemical impedance spectroscopy (EIS). A single rat fibroblast cell, or chloroplast purified from Peperomia metallica leaves, was immobilized by a micropipette after which the UME was inserted into its cytosolic space through cell membrane using a piezo actuator. An in vivo EIS measurement between the UME and the counter electrode outside of a single cell was taken. The measurements were analyzed using equivalent circuits in order to estimate the membrane impedance of a single cell. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：3094 / 3100

页数：7

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