Rapid HCP5 single-nucleotide polymorphism genotyping: A simple allele-specific PCR method for prediction of hypersensitivity reaction to Abacavir

被引：14

作者：

Galvan, Cristian A. ^{[1
,2
]}

Elbarcha, Osvaldo C. ^{[1
]}

Fernandez, Eduardo J. ^{[1
]}

Beltramo, Dante M. ^{[1
,3
]}

Walter Soria, Nestor ^{[1
,2
]}

机构：

[1] Lab Anal Clin Especializados LACE, RA-5000 Cordoba, Argentina

[2] Univ Catolica Cordoba, Fac Ciencias Quim, Catedra Biotecnol, Cordoba, Argentina

[3] Ctr Excelencia Prod & Proc Cordoba CEPROCOR, Cordoba, Argentina

来源：

CLINICA CHIMICA ACTA | 2011年 / 412卷 / 15-16期

关键词：

HCP5; polymorphism; Abacavir hypersensitivity; Pharmacogenetics; Genotyping; Allele specific PCR; HLA-B*5701; HLA-B-ASTERISK-5701; HLA; ASSOCIATION;

D O I：

10.1016/j.cca.2011.04.010

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: The most important factor limiting the success of an antiretroviral therapy is toxicity. The HLA-B*5701 allele is predictive of hypersensitivity reaction to Abacavir, and this gene is in a perfect linkage disequilibrium with the rs2395029 SNP present in the HCP5 gene. Methods: Genomic DNA was extracted from blood obtained from 201 unrelated healthy Argentinean volunteers. The DNA was subjected to an allele-specific PCR method. Sequencing was performed to validate the test results. Results: We were successful to amplify specific fragment of interest from the DNA samples. The method is easy, specific and reproducible. Conclusions: The application of this methodology is a rapid and simple method to detect the HCP5 polymorphism (rs2395029) previous to administration of Abacavir in patients with HIV infection. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1382 / 1384

页数：3

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