Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites

被引：7

作者：

Bailey, Dustin W. ^{[1
]}

Attia, Ziv ^{[1
]}

Reinert, Stephan ^{[1
,2
]}

Hulke, Brent S. ^{[3
]}

Kane, Nolan C. ^{[1
]}

机构：

[1] Univ Colorado, Ecol & Evolutionary Biol Dept, Boulder, CO 80309 USA

[2] Friedrich Alexander Univ Erlangen Nuremberg, Dept Biol, Div Biochem, D-91058 Erlangen, Germany

[3] USDA ARS, Sunflower & Plant Biol Res Unit, Edward T Schafer Agr Res Ctr, Fargo, ND 58102 USA

来源：

BIOTECHNIQUES | 2022年 / 72卷 / 03期

关键词：

CTAB; DNA extraction; secondary metabolites; Silphium; sodium metabisulfite; EXTRACTION PROTOCOL; PLANTS;

D O I：

10.2144/btn-2021-0050

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

METHOD SUMMARY A protocol for obtaining high-quality DNA extracted from plant tissue of species known to have interfering secondary metabolites is described. The Asteraceae are the largest plant family but among the least studied at the genome level. Our work investigated practical methods to reduce the influence of secondary metabolites - specifically, phenolic compounds - on the extraction of DNA from Silphium spp. This genus is in the Heliantheae tribe of Asteraceae that also includes sunflower (Helianthus annuus). Three methods were attempted in Silphium, with varying condition and age of the leaf sample. A modified cetyl trimethylammonium bromide (CTAB) method on young leaves resulted in the best DNA yield, with sufficient sample purity. No perceptible difference was observed between fresh and lyophilized samples for any extraction method or leaf age. These results provide an excellent basis for DNA extraction of difficult plant samples.

引用

页码：85 / 89

页数：5

共 19 条

[1] GENETIC VARIABILITY AMONG UMBU-CAJAZEIRA ACCESSIONS BY ISSR MARKERS [J].

Brito Santana, Ivonilda Barbosa ;

de Oliveira, Eder Jorge ;

Soares Filho, Walter dos Santos ;

Ritznger, Rogerio ;

Amorim, Edson Perito ;

de Carvalho Costa, Maria Angelica Pereira ;

Cunha Moreira, Ricardo Franco .

REVISTA BRASILEIRA DE FRUTICULTURA, 2011, 33 (03) :868-876

[2]

Byng JW, 2016, BOT J LINN SOC, V181, P1, DOI [10.1111/boj.12385, 10.1111/j.1095-8339.2009.00996.x]

[3] Incorporation of sodium sulfite into extraction protocol minimizes degradation of Acacia DNA [J].

Byrne, M ;

Macdonald, B ;

Francki, M .

BIOTECHNIQUES, 2001, 30 (04) :742-+

[4]

Friar EA, 2005, METHOD ENZYMOL, V395, P3

[5] Botanical characteristics, crop management and potential of Silphium perfoliatum L. as a renewable resource for biogas production: A review [J].

Gansberger, Markus ;

Montgomery, Lucy F. R. ;

Liebhard, Peter .

INDUSTRIAL CROPS AND PRODUCTS, 2015, 63 :362-372

[6]

Geneaid, 2021, GENEZOL TRADE DNA RE

[7]

Jacobs C.A., 1990, P 12 N AM PRAIR C CE, P33

[8]

Kowalski Radoslaw, 2004, Polish Journal of Food and Nutrition Sciences, V13, P349

[9]

Lehmkuhler, 2007, PLANT MANAGEMENT NET, V5, P1, DOI DOI 10.1094/FG-2007-1107-RS>

[10]

Lodhi M. A., 1994, Plant Molecular Biology Reporter, V12, P6, DOI 10.1007/BF02668658

← 1 2 →