Monitoring TFEB translocation

被引:4
作者
Chen, Guo [1 ]
Mu, Chenglong [1 ]
Chen, Yanfang [1 ]
An, Na [1 ]
Zhu, Yushan [1 ]
Kepp, Oliver [2 ,3 ]
Kroemer, Guido [2 ,3 ,4 ,5 ,6 ]
机构
[1] Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol, Tianjin, Peoples R China
[2] Univ Paris, Sorbonne Univ, Inserm U1138,Inst Univ France, Ctr Rech Cordeliers,Equipe Labellisee Ligue Canc, Paris, France
[3] Univ Paris Saclay, Gustave Roussy Comprehens Canc Inst, Metabol & Cell Biol Platforms, Villejuif, France
[4] Chinese Acad Med Sci, Suzhou Inst Syst Med, Suzhou, Peoples R China
[5] Hop Europeen Georges Pompidou, AP HP, Pole Biol, Paris, France
[6] Karolinska Univ Hosp, Karolinska Inst, Dept Womens & Childrens Hlth, Stockholm, Sweden
来源
MONITORING VESICULAR TRAFFICKING IN CELLULAR RESPONSES TO STRESS - PT A | 2021年 / 164卷
关键词
REGULATING LYSOSOMAL BIOGENESIS; TRANSCRIPTION FACTORS; AUTOPHAGY;
D O I
10.1016/bs.mcb.2020.10.017
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The transcription factor EB (TFEB) plays a critical role in autophagy induction and lysosomal biogenesis by orchestrating the expression of autophagy- and lysosome-related genes. In response to a series of stresses such as nutrient starvation, TFEB translocates from the cytoplasm to the nucleus, where it exerts its regulatory function. The activity of TFEB is tightly regulated by multiple phosphorylation and acetylation sites. Methods that rely on the analysis of posttranslational modification as a proxy for TFEB activation are often misleading. Here, we elaborate on protocols for monitoring nuclear translocation of TFEB by fluorescence microscopy.
引用
收藏
页码:1 / 9
页数:9
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