Screening, confirmation and quantitation of diuretics in urine for doping control analysis by high-performance liquid chromatography-atmospheric pressure ionisation tandem mass spectrometry

被引：62
作者：
Thieme, D [1 ]
Grosse, J [1 ]
Lang, R [1 ]
Mueller, RK [1 ]
Wahl, A [1 ]
机构：
[1] Inst Doping Anal & Sports Biochem, D-01731 Kreischa, Germany
来源：
JOURNAL OF CHROMATOGRAPHY B | 2001年 / 757卷 / 01期
关键词：
doping control; diuretics; benzothiadiazine;
D O I：
10.1016/S0378-4347(01)00058-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive, selective, robust and fast method to identify 32 diuretics and masking agents in mine is described. The analytical procedure is reduced to a single XAD extraction step for sample preparation, followed by reversed-phase liquid chromatography in combination with atmospheric pressure ionisation/tandem mass spectrometry. This technique is, after minor modifications, suitable for screening analyses and confirmation of identity as well as quantitation of diuretics. Considerations relating to the stability and metabolism of the compounds are given if relevant for routine screening analyses. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：49 / 57
页数：9
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