Screening, confirmation and quantitation of diuretics in urine for doping control analysis by high-performance liquid chromatography-atmospheric pressure ionisation tandem mass spectrometry

被引：62

作者：

Thieme, D ^{[1
]}

Grosse, J ^{[1
]}

Lang, R ^{[1
]}

Mueller, RK ^{[1
]}

Wahl, A ^{[1
]}

机构：

[1] Inst Doping Anal & Sports Biochem, D-01731 Kreischa, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 757卷 / 01期

关键词：

doping control; diuretics; benzothiadiazine;

D O I：

10.1016/S0378-4347(01)00058-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive, selective, robust and fast method to identify 32 diuretics and masking agents in mine is described. The analytical procedure is reduced to a single XAD extraction step for sample preparation, followed by reversed-phase liquid chromatography in combination with atmospheric pressure ionisation/tandem mass spectrometry. This technique is, after minor modifications, suitable for screening analyses and confirmation of identity as well as quantitation of diuretics. Considerations relating to the stability and metabolism of the compounds are given if relevant for routine screening analyses. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：49 / 57

页数：9

共 20 条

[1] DETERMINATION AND IDENTIFICATION OF AMILORIDE IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY
BI, HG
COOPER, SF
COTE, MG
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 582 (1-2): : 93 - 101
[2] HPLC atmospheric pressure chemical ionization mass spectroscopy of eight regulated sulfonamides
Combs, MT
Ashraf-Khorassani, M
Taylor, LT
[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1999, 19 (3-4) : 301 - 308
[3] Determination of sulfonamides by packed column supercritical fluid chromatography with atmospheric pressure chemical ionisation mass spectrometric detection
Dost, K
Jones, DC
Davidson, G
[J]. ANALYST, 2000, 125 (07) : 1243 - 1247
[4] Garbis SD, 1998, J AOAC INT, V81, P948
[5] Gergov M, 2000, J MASS SPECTROM, V35, P912, DOI 10.1002/1096-9888(200007)35:7<912::AID-JMS19>3.0.CO
[6] 2-4
[7] Direct determination of diuretic drugs in urine by capillary zone electrophoresis using fluorescence detection
Gonzalez, E
Becerra, A
Laserna, JJ
[J]. JOURNAL OF CHROMATOGRAPHY B, 1996, 687 (01): : 145 - 150
[8] ANALYSIS OF BUMETANIDE IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
GRADEEN, CY
BILLAY, DM
CHAN, SC
[J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 1990, 14 (02) : 123 - 126
[9] Fast liquid chromatographic-mass spectrometric determination of pharmaceutical compounds
Heinig, K
Henion, J
[J]. JOURNAL OF CHROMATOGRAPHY B, 1999, 732 (02): : 445 - 458
[10] Rapid screening for diuretic doping agents in urine by C60-assisted laser-desorption-ionization-time-of-flight mass spectrometry
Huang, JP
Yuan, CH
Shiea, J
Chen, YC
[J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 1999, 23 (05) : 337 - 342

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