Identification of sex linked molecular markers in spine gourd (Momordica dioica Roxb.)

被引：2
|
作者：
Ameen, Gajala [1 ]
Tiwari, Jitendra Kumar [1 ]
Prakash, Ved [1 ]
Sandilya, Vivek Kumar [1 ]
Das, B. K. [2 ]
机构：
[1] Indira Gandhi Krishi Vishwavidyalaya, Raj Mohini Devi Coll Agr & Res Stn, Sect Genet & Plant Breeding, Ambikapur 497001, Chhattisgarh, India
[2] Bhabha Atom Res Ctr, Nucl Agr & Biotechnol Div, DAE, Mumbai 400085, Maharashtra, India
来源：
INDIAN JOURNAL OF GENETICS AND PLANT BREEDING | 2022年 / 82卷 / 01期
关键词：
DNA extraction; Momordica dioica; PCR amplification; simple sequence repeat (SSR); CUCUMBER;
D O I：
10.31742/IJGPB.82.1.17
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The major drawback of a dioecious spine gourd (Momordica dioica Roxb.) is distinguishing whether the plant is male or female at an earlier development stage. An investigation was aimed to eliminate aforesaid limitation by identifying and validating sex linked molecular marker. Twenty six male and female genotypes of each sex were screened with 12 specific SSR primers. Only two SSR primers, MdSSR 5 (MADS-box gene of spine gourd) and CMbSSR 6 (MADS-box gene of cucumber) showed amplification and polymorphism in female genotypes only. These specific female linked markers manifest existent of non-epigenetic factors entailed in gender development in the spine gourd.
引用
收藏
页码：113 / 115
页数：3
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