Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay

被引：41

作者：

Machado, ER

Ueta, MT

Gonçalves-Pires, MDD

de Oliveira, JBA

Faccioli, LH

Costa-Cruz, JM

机构：

[1] Univ Fed Uberlandia, Parasitol Lab, Dept Imunol Microbiol & Parasitol, Inst Ciencias Biomed, BR-38400902 Uberlandia, MG, Brazil

[2] Univ Estadual Campinas, Inst Biol, Dept Parasitol, Campinas, SP, Brazil

[3] Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxol & Bromatol, Lab Imunol, Ribeirao Preto, SP, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2003年 / 98卷 / 06期

关键词：

Strongyloides venezuelensis; strongyloidiasis; diagnosis; enzyme-linked immunosorbent assay;

D O I：

10.1590/S0074-02762003000600024

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). Results were expressed in antibody titers, which were considered as positive when titer was greater than or equal to80. Sensibility and specificity of the assay were 100% and 96.7%, respectively. It can be concluded that the heterologous alkaline extract could be employed in ELISA as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity.

引用

页码：849 / 851

页数：3

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