Chrysanthemum indicum suppresses adipogenesis by inhibiting mitotic clonal expansion in 3T3-L1 preadipocytes

被引:9
作者
Kim, Won-Ju [1 ]
Yu, Hyung-Seok [1 ]
Bae, Won-Young [1 ]
Ko, Kyung Yuk [2 ]
Chang, Kyung-Hoon [2 ,3 ]
Lee, Na-Kyoung [1 ]
Paik, Hyun-Dong [1 ]
机构
[1] Konkuk Univ, Dept Food Sci & Biotechnol Anim Resources, Seoul 05029, South Korea
[2] Natl Inst Food & Drug Safety Evaluat, Osong, South Korea
[3] CJ CheilJedang Blossom Pk, Gyeonggi Do, South Korea
关键词
adipogenesis; Akt signaling pathway; Chrysanthemum indicum; ERK; 1; 2 signaling pathway; mitotic clonal expansion; EARLY-STAGE; DIFFERENTIATION; EXTRACTS; AMPK;
D O I
10.1111/jfbc.13896
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Herbs have been of interest to treat diseases, including obesity, owing to their various bioactive constituents that exhibit therapeutic and prophylactic properties. The present study examined the anti-adipogenic effects and mechanisms of Chrysanthemum indicum aqueous extract (CAE) in 3T3-L1 preadipocytes. CAE comprises 1,3-dicaffeoylquinic acid, chlorogenic acid, kaempferol-3-O-glucoside, caffeic acid, and apigenin, which were corresponded with previous reports. CAE inhibited the accumulation of lipid droplets and significantly alleviated the expression of lipogenesis- and adipogenesis-associated biomarkers. Treatment with CAE inhibited the mitotic clonal expansion (MCE), corroborated by cell cycle arrest at the G(0)/G(1) phase, and mitigated the expression of cell cycle progression-associated proteins and in addition to phosphorylation of MCE-promoting transcription factors. Moreover, CAE downregulated the activation of Akt and extracellular signal-regulated kinase 1/2 signaling pathways. In summary, CAE facilitates adipogenic inhibition during the early phase of differentiation, especially MCE, and its phenolic compounds can contribute to its anti-obesogenic properties. Practical applications Chrysanthemum indicum has been mainly used as traditional herbal tea and drinks. Chrysanthemum indicum aqueous extract (CAE) inhibits adipogenesis by suppressing mitotic clonal expansion during the early phase of differentiation in 3T3-L1 preadipocytes. 1,3-Dicaffeoylquinic acid, chlorogenic acid, kaempferol-3-O-glucoside, caffeic acid, and apigenin were detected in CAE. Based on these findings, CAE can be used as nutraceutical agents for prevention and treatment of obesity.
引用
收藏
页数:11
相关论文
共 50 条
[41]   Chrysanthemum morifoliumFlower Extract Inhibits Adipogenesis of 3T3-L1 Cells via AMPK/SIRT1 Pathway Activation [J].
Lee, Mak-Soon ;
Kim, Yangha .
NUTRIENTS, 2020, 12 (09) :1-12
[42]   Melatonin Induces Apoptotic Cell Death in 3T3-L1 Preadipocytes [J].
Lee, J. ;
Yoo, Y. -M. ;
Lee, Y. H. ;
Kim, C. H. .
MOLECULAR BIOLOGY, 2020, 54 (02) :204-212
[43]   Histone demethylase Kdm4b functions as a co-factor of C/EBPβ to promote mitotic clonal expansion during differentiation of 3T3-L1 preadipocytes [J].
Guo, L. ;
Li, X. ;
Huang, J-X ;
Huang, H-Y ;
Zhang, Y-Y ;
Qian, S-W ;
Zhu, H. ;
Zhang, Y-D ;
Liu, Y. ;
Liu, Y. ;
Wang, K-K ;
Tang, Q-Q .
CELL DEATH AND DIFFERENTIATION, 2012, 19 (12) :1917-1927
[44]   Illudins C2 and C3 Stimulate Lipolysis in 3T3-L1 Adipocytes and Suppress Adipogenesis in 3T3-L1 Preadipocytes [J].
Kim, Sun-Ok ;
Sakchaisri, Krisada ;
Asami, Yukihiro ;
Ryoo, In-Ja ;
Choo, Soo-Jin ;
Yoo, Ick-Dong ;
Soung, Nak-Kyun ;
Kim, Young Sang ;
Jang, Jae-Hyuk ;
Kim, Bo Yeon ;
Ahn, Jong Seog .
JOURNAL OF NATURAL PRODUCTS, 2014, 77 (04) :744-750
[45]   Anacardic Acid Suppresses Adipogenesis Through Inhibition of the Hsp90/Akt Signaling Pathway in 3T3-L1 Preadipocytes [J].
Lee, Jangho ;
Chung, Min-Yu ;
Chung, Sangwon ;
Choi, Hyo-Kyoung .
JOURNAL OF MEDICINAL FOOD, 2021, 24 (05) :487-496
[46]   Lysine Deprivation Suppresses Adipogenesis in 3T3-L1 Cells: A Transcriptome Analysis [J].
Lee, Leo Man-Yuen ;
Lin, Zhi-Qiang ;
Zheng, Lu-Xi ;
Tu, Yi-Fan ;
So, Yik-Hing ;
Zheng, Xiu-Hua ;
Feng, Tie-Jun ;
Wang, Xi-Yue ;
Wong, Wai-Ting ;
Leung, Yun-Chung .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (11)
[47]   Palmitoyl lactic acid induces adipogenesis and a brown fat-like phenotype in 3T3-L1 preadipocytes [J].
Unno, Yuka ;
Yamamoto, Hirona ;
Takatsuki, Shuto ;
Sato, Yoshinori ;
Kuranaga, Takefumi ;
Yazawa, Kazunaga ;
Ono, Yasuo ;
Wakimoto, Toshiyuki .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2018, 1863 (07) :772-782
[48]   Cyanidin inhibits adipogenesis in 3T3-L1 preadipocytes by activating the PLC-IP3 pathway [J].
Kongthitilerd, Phutthida ;
Barras, Elise ;
Rong, Weiqiong ;
Thibodeaux, Ansleigh ;
Rigdon, Madison ;
Yao, Shaomian ;
Adisakwattana, Sirichai ;
Suantawee, Tanyawan ;
Cheng, Henrique .
BIOMEDICINE & PHARMACOTHERAPY, 2023, 162
[49]   Perfluorobutanesulfonic acid (PFBS) potentiates adipogenesis of 3T3-L1 adipocytes [J].
Qi, Weipeng ;
Clark, John M. ;
Timme-Laragy, Alicia R. ;
Park, Yeonhwa .
FOOD AND CHEMICAL TOXICOLOGY, 2018, 120 :340-345
[50]   Neptunea cumingii Extract Inhibits Adipogenesis and Stimulates Browning of Adipocytes in 3T3-L1 Preadipocytes [J].
Dayarathne, Lakshi A. ;
Ko, Seok-Chun ;
Yim, Mi-Jin ;
Lee, Jeong Min ;
Kim, Ji-Yul ;
Oh, Gun-Woo ;
Lee, Dae-Sung ;
Jung, Won-Kyo ;
Lee, Sei-Jung ;
Je, Jae-Young .
JOURNAL OF FOOD BIOCHEMISTRY, 2025, 2025 (01)