Differential regulation of groESL operon expression in response to heat and light in Anabaena

被引：26

作者：

Rajaram, Hema ^{[1
]}

Apte, Kumar ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr, Div Mol Biol, Bombay 400085, Maharashtra, India

来源：

ARCHIVES OF MICROBIOLOGY | 2010年 / 192卷 / 09期

关键词：

Heat-regulation; HrcA; CIRCE; groESL; cpn60; Anabaena; NITROGEN-FIXING CYANOBACTERIUM; SYNECHOCYSTIS-SP PCC-6803; SP STRAIN PCC-6803; SHOCK RESPONSE; BACILLUS-SUBTILIS; CHAPERONIN MACHINE; MAJOR MODULATOR; DNA-BINDING; GENE; HRCA;

D O I：

10.1007/s00203-010-0601-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The HrcA protein is known to bind the cis-element CIRCE and repress expression of hsp60 in certain bacteria. However, recent data from cyanobacteria have seriously questioned the HrcA/CIRCE interaction paradigm. A hrcA null mutant showed constitutive expression of Hsp60 proteins [GroEL/Cpn60(GroEL2)], and an unexpected further increase in GroEL during temperature upshift, suggesting involvement of regulatory mechanisms other than HrcA in groESL expression in Anabaena. The negative regulation of both hsp60 genes [groEL and cpn60 (groEL2)] at CIRCE element was established by: (1) constitutive expression of Green Fluorescent Protein gene, tagged to Anabaena hsp60 promoters, in E. coli, and its repression upon co-expression of Anabaena HrcA and (2) specific binding of Anabaena HrcA to the CIRCE element. Deletion analysis of other cis-elements further distinguished (a) a photo-regulation by the K-box and (b) thermoregulation from a novel H-box, over and above the negative regulation by HrcA at CIRCE.

引用

页码：729 / 738

页数：10

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