The self-assembled gold nanoparticle film deposited from the liquid-liquid interface has been fabricated, and homogeneous surfaceenhanced Raman scattering (SERS) of R6G molecules on the film has been observed. The SERS enhancement factor of the film is estimated to be in the range 10(8)-10(10). Occasionally, there are some sudden jumps in the Raman signal, where the signal is increased a few times. Such jumps are probably caused by single-molecule SERS in some extremely "hot" sites. (c) 2004 Elsevier B.V. All rights reserved.