Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid

被引：15
|
作者：
Xu, Xiaomin [1 ]
But, Andrada [1 ]
Wever, Ron [2 ]
Hollmann, Frank [1 ]
机构：
[1] Delft Univ Technol, Dept Biotechnol, van der Maasewg 9, NL-2629 HZ Delft, Netherlands
[2] Univ Amsterdam, Vant Hoff Inst Mol Sci, NL-1098 XH Amsterdam, Netherlands
来源：
CHEMCATCHEM | 2020年 / 12卷 / 08期
基金：
欧洲研究理事会; 欧盟地平线“2020”;
关键词：
glutamic acid; biocatalysis; vanadium chloroperoxidase; nitriles; oxidative decarboxylation; VANADIUM CHLOROPEROXIDASE; AMINO-ACIDS; GREEN; BIOCATALYSIS;
D O I：
10.1002/cctc.201902194
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The chemoenzymatic oxidative decarboxylation of glutamic acid to the corresponding nitrile using the vanadium chloroperoxidase from Curvularia inaequalis (CiVCPO) as HOBr generation catalysts has been investigated. Product inhibition was identified as major limitation. Nevertheless, 1630000 turnovers and k(cat) of 75 s(-1) were achieved using 100 mM glutamate. The semi-preparative enzymatic oxidative decarboxylation of glutamate was also demonstrated.
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页码：2180 / 2183
页数：4
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