Microcalorimetric and spectroscopic investigation of the antibacterial properties of cationic Ytterbium(III)-porphyrin complexes lacking charged peripheral groups

被引：11
作者：
Hou, An-Xin [1 ]
Xue, Zhi [1 ]
Liu, Yi [1 ]
Qu, Song-Sheng [1 ]
Wong, Wai-Kwok [1 ,2 ]
机构：
[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
[2] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China
来源：
CHEMISTRY & BIODIVERSITY | 2007年 / 4卷 / 12期
关键词：
D O I：
10.1002/cbdv.200790238
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The antibacterial activities towards Escherichia coli of two cationic Yb-III-monoporphyrin complexes, [Yb-III(TMP)(H2O)(3)]Cl (1) and [Yb-III(TTP)(H2O)(3)]Cl (2), were investigated at the cellular and sub-cellular levels. The biological effects of the complexes on the growth of E. coli were evaluated by microcalorimetry and by analysis of the resulting metabolic thermogenic curves, from which IC50 values and metabolic parameters such as growth rate and generation time were derived. At the subcellular level, DNA-binding experiments were performed by means of UV/VIS- and fluorescence-titration experiments, as well as by near-infrared (NIR) emission, which revealed that 1 and 2 strongly bind to herring-sperm DNA (HS-DNA), though by different binding modes.
引用
收藏
页码：2889 / 2899
页数：11
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