Quorum Sensing Inhibitors of Staphylococcus aureus from Italian Medicinal Plants

被引:41
作者
Quave, Cassandra L. [1 ]
Plano, Lisa R. W. [2 ]
Bennett, Bradley C. [1 ]
机构
[1] Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA
[2] Univ Miami, Miller Sch Med, Dept Microbiol & Immunol, Miami, FL 33136 USA
关键词
quorum sensing; MRSA; medicinal plants; delta-toxin; delta-hemolysin; agr; MULTIDRUG EFFLUX PUMPS; VIRULENCE; ANTIBACTERIAL; INFECTIONS; RESISTANCE; ALBANIANS; ARBERESHE; TARGET; MRSA;
D O I
10.1055/s-0030-1250145
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Morbidity and mortality estimates due to methicillin-resistant Staphylococcus aureus (MRSA) infections continue to rise. Therapeutic options are limited by antibiotic resistance. Anti-pathogenic compounds, which inhibit quorum sensing (QS) pathways, may be a useful alternative to antibiotics. Staphylococcal QS is encoded by the agr locus and is responsible for the production of delta-hemolysin. Quantification of delta-hemolysin found in culture supernatants permits the analysis of agr activity at the translational rather than transcriptional level. We employed reversed phase high performance chromatographic (RP-HPLC) techniques to investigate the anti-QS activity of 168 extracts from 104 Italian plants through quantification of delta-hemolysin. Extracts from three medicinal plants (Ballota nigra, Castanea sativa, and Sambucus ebulus) exhibited a dose-dependent response in the production of delta-hemolysin, indicating anti-QS activity in a pathogenic MRSA isolate.
引用
收藏
页码:188 / 195
页数:8
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