Influence of auxins, cytokinins, and nitrogen on production of rutin from callus and adventitious roots of the white mulberry tree (Morus alba L.)

被引:63
作者
Lee, Yew [1 ]
Lee, Dong-Eun [1 ]
Lee, Hak-Soo [1 ]
Kim, Seong-Ki [2 ]
Lee, Woo Sung [3 ]
Kim, Soo-Hwan [1 ]
Kim, Myoung-Won [1 ]
机构
[1] Yonsei Univ, Dept Biol Sci & Technol, Wonju 220710, South Korea
[2] Chung Ang Univ, Dept Life Sci, Seoul 156756, South Korea
[3] Sungkyunkwan Univ, Dept Biol Sci, Suwon 440746, South Korea
关键词
Adventitious roots; Auxin; Callus; Cytokinin; Mulberry plants; NH4+-to-NO3- ratio; Plant growth regulator; Rutin; Secondary metabolites; CELL-SUSPENSION CULTURES; SECONDARY METABOLITES; HAIRY ROOTS; PHYTOLACCA-AMERICANA; CATHARANTHUS-ROSEUS; TISSUE CULTURES; SHOOT CULTURES; PLANT-CELLS; ACCUMULATION; NITRATE;
D O I
10.1007/s11240-010-9832-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Rutin is an economically valuable flavone compound with anticancer activity, dietary effects, and anti-aging activity. In this study, callus and adventitious roots were induced from three Morus (mulberry) species. Among the three mulberry species tested for rutin production, roots of the Sugye (M. alba L.) had the highest levels (242.2 mu g/g fresh tissue) of rutin. In addition, the mature leaves of this type of tree promoted higher levels of rutin compared to those of young leaves or those undergoing senescence. Adding auxins such as indole-3-acetic acid (IAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (NAA) not only enhanced the development of callus and adventitious roots but also increased the protein and rutin contents. In contrast, adding cytokinins such as 6-benzyladenine (BA) and kinetin (KN) retarded callus and adventitious root development as well as the protein and rutin contents. Callus in suspension culture in the presence of IAA produced more rutin than that in the absence of IAA. However, rutin secretion into a medium was greater in the absence of IAA. Different ammonium/nitrate (AM/NI) ratios in a root suspension culture also greatly affected rutin production and its secretion into a liquid medium. As a result, the highest level of rutin was produced when adventitious roots were grown in a 34/66 AM/NI full-strength standard MS medium containing 5 mg/l IAA.
引用
收藏
页码:9 / 19
页数:11
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