Accessing depth-resolved high spatial frequency content from the optical coherence tomography signal

被引:7
作者
Alexandrov, Sergey [1 ]
Arangath, Anand [1 ]
Zhou, Yi [1 ]
Murphy, Mary [2 ]
Duffy, Niamh [2 ]
Neuhaus, Kai [1 ]
Shaw, Georgina [2 ]
McAuley, Ryan [1 ]
Leahy, Martin [1 ,3 ]
机构
[1] Natl Univ Ireland, Natl Biophoton & Imaging Platform, Sch Phys, Tissue Opt & Microcirculat Imaging Grp, Galway H91 TK33, Ireland
[2] Natl Univ Ireland, Sch Med, Regenerat Med Inst, Galway, Ireland
[3] Inst Photon Sci ICFO, Barcelona, Spain
基金
欧盟地平线“2020”;
关键词
MESENCHYMAL STEM-CELLS; CHONDROGENIC DIFFERENTIATION; OCT;
D O I
10.1038/s41598-021-96619-7
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Optical coherence tomography (OCT) is a rapidly evolving technology with a broad range of applications, including biomedical imaging and diagnosis. Conventional intensity-based OCT provides depth-resolved imaging with a typical resolution and sensitivity to structural alterations of about 5-10 microns. It would be desirable for functional biological imaging to detect smaller features in tissues due to the nature of pathological processes. In this article, we perform the analysis of the spatial frequency content of the OCT signal based on scattering theory. We demonstrate that the OCT signal, even at limited spectral bandwidth, contains information about high spatial frequencies present in the object which relates to the small, sub-wavelength size structures. Experimental single frame imaging of phantoms with well-known sub-micron internal structures confirms the theory. Examples of visualization of the nanoscale structural changes within mesenchymal stem cells (MSC), which are invisible using conventional OCT, are also shown. Presented results provide a theoretical and experimental basis for the extraction of high spatial frequency information to substantially improve the sensitivity of OCT to structural alterations at clinically relevant depths.
引用
收藏
页数:10
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