Mechanical loading regulates expression of talin and its mRNA, which are concentrated at myotendinous junctions

被引:25
作者
Frenette, J [1 ]
Tidball, JG [1 ]
机构
[1] Univ Calif Los Angeles, Dept Physiol Sci, Los Angeles, CA 90095 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1998年 / 275卷 / 03期
关键词
rat; soleus muscle; cyclic loading; C2C12; myotubes;
D O I
10.1152/ajpcell.1998.275.3.C818
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The hypothesis that mechanical loading regulates talin expression in developing and adult muscle was tested using in vitro and in vivo models. Talin was selected for study because it is a key structural link between the cytoskeleton and cell membrane. In the in vitro model, C2C12 myotubes were subjected to cyclic strains for 48 h. In the in vivo model, rat hindlimb muscles were unloaded for 10 days, then reloaded for 2 days. Cyclic loading of myotubes resulted in significant increases in the quantity of talin (68%) and its 190-kDa proteolytic fragment (70%), as well as talin mRNA (180%), relative to unloaded myotube cultures. Similarly, talin concentration and its mRNA increased by 68 and 136%, respectively, in soleus muscles reloaded for 2 days relative to ambulatory controls. Immunohistochemistry and in situ RT-PCR showed that talin and its mRNA are concentrated and colocalized at myotendinous junctions. Thus these findings indicate that increased mechanical loading promotes talin synthesis, which occurs principally at myotendinous junctions, according to talin mRNA distribution.
引用
收藏
页码:C818 / C825
页数:8
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