Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597

被引：5

作者：

Sugiyama, Yosuke ^{[1
]}

Senda, Miki ^{[2
]}

Senda, Toshiya ^{[2
]}

Matsuda, Tomoko ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Bioengn, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[2] High Energy Accelerator Res Org KEK, Photon Factory, Inst Mat Struct Sci, Struct Biol Res Ctr, Tsukuba, Ibaraki 3050801, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2015年 / 71卷

关键词：

acetophenone reductase; enantioselectivity; asymmetric reduction; SUPERCRITICAL CARBON-DIOXIDE; ALCOHOL-DEHYDROGENASE; ASYMMETRIC REDUCTION; KETONES; PARAPSILOSIS;

D O I：

10.1107/S2053230X15002265

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetophenone reductase (APRD) from Geotrichum candidium NBRC 4597 was crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as a precipitant. The crystal belonged to space group P6522, with unit-cell parameters a = b = 104.5, c = 273.7 angstrom, and diffracted to 2.6 angstrom resolution. Phasing using the single-wavelength anomalous diffraction method was successful. Model building and crystallographic refinement are in progress.

引用

页码：320 / 323

页数：4

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