Unexpected complexation reaction during analysis of proteins using laser desorption spray post-ionization mass spectrometry

被引：9

作者：

Liu, Jia ^{[1
]}

Zhang, Chengsen ^{[1
]}

Sun, Jiamu ^{[1
]}

Luo, Hai ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China

来源：

ANALYST | 2012年 / 137卷 / 08期

基金：

中国国家自然科学基金;

关键词：

ABLATION ELECTROSPRAY-IONIZATION; ATMOSPHERIC-PRESSURE; AMBIENT CONDITIONS; SOLIDS;

D O I：

10.1039/c2an16090k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe complexation reactions of insulin and other proteins with metal ions generated from the substrate surface by laser irradiation in laser desorption spray post-ionization mass spectrometry (LDSPI-MS). This particular type of complexation reaction in LDSPI-MS was investigated for the first time, which indicated that the mechanistic process of LDSPI-MS might be much more complicated than that proposed before for similar methods.

引用

页码：1764 / 1767

页数：4

共 20 条

[1] Analysis of Amphiphilic Lipids and Hydrophobic Proteins Using Nonresonant Femtosecond Laser Vaporization with Electrospray Post-Ionization [J].

Brady, John J. ;

Judge, Elizabeth J. ;

Levis, Robert J. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2011, 22 (04) :762-772

[2] Electrospray-assisted laser desorption/ionization mass spectrometry for continuously monitoring the states of ongoing chemical reactions in organic or aqueous solution under ambient conditions [J].

Cheng, Chi-Yuan ;

Yuan, Cheng-Hui ;

Cheng, Sy-Chyi ;

Huang, Min-Zong ;

Chang, Hui-Chiu ;

Cheng, Tien-Lu ;

Yeh, Chen-Sheng ;

Shiea, Jentaie .

ANALYTICAL CHEMISTRY, 2008, 80 (20) :7699-7705

[3] Nanomaterial-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry of Peptides and Proteins [J].

Chiang, Cheng-Kang ;

Chiang, Ni-Chen ;

Lin, Zong-Hong ;

Lan, Guo-Yu ;

Lin, Yang-Wei ;

Chang, Huan-Tsung .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2010, 21 (07) :1204-1207

[4] Study of the ionization mechanism in hybrid laser based desorption techniques [J].

Dixon, R. Brent ;

Muddiman, David C. .

ANALYST, 2010, 135 (05) :880-882

[5] Formation and characterization of iron-oligonucleotide complexes with matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry [J].

Hettich, RL .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1999, 10 (10) :941-949

[6] Characterization of the chemical components on the surface of different solids with electrospray-assisted laser desorption ionization mass spectrometry [J].

Huang, Min-Zong ;

Hsu, Hsiu-Jung ;

Wu, Chen-I ;

Lin, Shu-Yao ;

Ma, Ya-Lin ;

Cheng, Tian-Lu ;

Shiea, Jentaie .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007, 21 (11) :1767-1775

[7] Effects of matrix, electrospray solution, and laser light on the desorption and ionization mechanisms in electrospray-assisted laser desorption ionization mass spectrometry [J].

Huang, Min-Zong ;

Jhang, Siou-Sian ;

Cheng, Chun-Nian ;

Cheng, Sy-Chyi ;

Shiea, Jentaie .

ANALYST, 2010, 135 (04) :759-766

[8] LASER DESORPTION IONIZATION OF PROTEINS WITH MOLECULAR MASSES EXCEEDING 10000 DALTONS [J].

KARAS, M ;

HILLENKAMP, F .

ANALYTICAL CHEMISTRY, 1988, 60 (20) :2299-2301

[9] Atmospheric pressure matrix assisted laser desorption/ionization mass spectrometry [J].

Laiko, VV ;

Baldwin, MA ;

Burlingame, AL .

ANALYTICAL CHEMISTRY, 2000, 72 (04) :652-657

[10] Fingerprinting of yogurt products by laser desorption spray post-ionization mass spectrometry [J].

Liu, Jia ;

Qiu, Bo ;

Luo, Hai .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2010, 24 (09) :1365-1370

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