FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5

被引：302

作者：

Jia, Xiaotong ^{[1
]}

Chen, Qiangqiang ^{[2
]}

Yang, Yingfang ^{[2
]}

Tang, Yao ^{[1
]}

Wang, Rui ^{[2
]}

Xu, Yufang ^{[1
]}

Zhu, Weiping ^{[1
]}

Qian, Xuhong ^{[1
]}

机构：

[1] East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, State Key Lab Bioreactor Engn, Meilong Rd 130, Shanghai 200237, Peoples R China

[2] East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Meilong Rd 130, Shanghai 200237, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2016年 / 138卷 / 34期

基金：

中国国家自然科学基金;

关键词：

NITRIC-OXIDE; SELECTIVE DETECTION; TYROSINE NITRATION; LIVING CELLS; REDOX CYCLES; IN-VIVO; SUPEROXIDE; DYES;

D O I：

10.1021/jacs.6b06398

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Peroxynitrite (OONO-) is profoundly implicated in health and disease. The physiological and pathological outcome of OONO- is related to its local Concentration, and hence, a reliable OONO- assay is highly desired. We have developed a FRET-based small molecule fluorescent probe (PNCy3Cy5), harnessing the differential reactivity of Cy3 and Cy5 toward OONO- by fine-tuning. It exhibits high detection sensitivity and yields a ratiometric fluorescent signal. We have exemplified that it can be applied in semiquantitative determination of OONO- in living cells. Notably, it specifically localizes in mitochondria where endogenous OONO- is predominantly generated. Thus, PNCy3Cy5 is a promising molecular tool for peroxynitrite biology.

引用

页码：10778 / 10781

页数：4

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