Effect of patchouli alcohol on Helicobacter pylori-induced neutrophil recruitment and activation

被引：13

作者：

Ren, Wen-Kang ^{[1
]}

Xu, Yi-Fei ^{[1
]}

Wei, Wen-Hui ^{[1
,2
]}

Huang, Ping ^{[1
,3
]}

Lian, Da-Wei ^{[1
]}

Fu, Li-Jun ^{[1
]}

Yang, Xu-Feng ^{[1
]}

Chen, Fang-Jun ^{[1
]}

Wang, Jing ^{[1
]}

Cao, Hong-Ying ^{[1
]}

Deng, Yuan-Hui ^{[4
]}

机构：

[1] Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, 232 Wai Huan East Rd, Guangzhou 510006, Guangdong, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China

[3] Dongguan & Guangzhou Univ Chinese Med, Cooperat Acad Math Engn Chinese Med, Dongguan 523808, Peoples R China

[4] Guangzhou Univ Chinese Med, Affiliated Hosp 2, 55 Nei Huan West Rd, Guangzhou 510006, Guangdong, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2019年 / 68卷

基金：

美国国家科学基金会;

关键词：

Patchouli alcohol; H; pylori; Air pouch model; Neutrophil; Extracellular ROS; p22/p47-phoxes; NADPH OXIDASE; INFLAMMATORY RESPONSE; GENE-EXPRESSION; SABA ADHESIN; KAPPA-B; HP-NAP; INFECTION; MUCOSAL; PATHOGENESIS; INDUCTION;

D O I：

10.1016/j.intimp.2018.12.044

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Neutrophil infiltration typically occurs in Helicobacter pylori (H. pylori)-induced acute gastritis; however, this immune response fails to eradicate H. pylori in vivo. Moreover, reactive oxygen species (ROS), which are generated by neutrophils, cause severe damage to gastric mucosa. Patchouli alcohol (PA) has been reported to have effective anti-oxidative and anti-H. pylori activities, and we investigated its effects on H. pylori-induced neutrophil recruitment and activation in this research. In neutrophil recruitment experiment, H. pylori was injected into rat air pouch to explore the effects of PA (10, 20 and 40 mg/kg) on acute inflammatory response. The results revealed that PA significantly reduced the weight of exudate and the number of neutrophils in the air pouch. Meanwhile, remarkable decrements in TNF-alpha and IL-8 levels in exudates were observed. In neutrophil activation experiment, rat neutrophils were isolated and activated by using 50 mu g/mL H. pylori water-soluble surface protein with or without the treatment of PA (5, 10 or 20 mu mol/L). Results indicated that PA not only significantly inhibited the production of ROS, but also reduced the gene and protein expressions of p22/p47-phoxes, and the binding of p22/p47-phoxes. Furthermore, the influence of PA on the neutrophil activation genes of H. pylori (h-nap and sabA) was investigated, and the results showed that expressions of h-nap and sabA were remarkably decreased after PA treatment. In conclusion, PA reduced the recruitment and activation of neutrophils induced by H. pylori, as shown by its inhibition of pro-inflammatory factor generation, p22/p47-phoxes function and H. pylori neutrophil activation-related gene expression.

引用

页码：7 / 16

页数：10

共 43 条

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