Kordiimonas lipolytica sp nov., isolated from seawater

A Gram-stain-negative, aerobic, rod-shaped bacterium, designated M41(T), was isolated from a surface seawater sample collected from the western Pacific Ocean. The isolate grew in medium containing 0.5-10.0 % (w/v) NaCl (optimally at 1.0-3.0 %) at 15-45 degrees C and pH 5.5-9.5. Positive for oxidase, catalase and nitrate reduction. The respiratory quinone is Q-10. The major fatty acids (>10 %) are iso-C-15:0, iso-C-17:1 omega 9c and summed feature 3 (comprising C-16:1 omega 7c and/or iso-C-15:0 2-OH). The major polar lipids are phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid, and three unidentified glycolipids. The genomic DNA G + C content is 56.3 mol %. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M41(T) should be assigned to the genus Kordiimonas. The 16S rRNA gene sequence similarities between the isolate and the type strains of species of the genus Kordiimonas with validly published names were in the range 96.2-98.6 %. Strain M41(T) exhibited average nucleotide identity (ANI) values of 81.7 and 72.3 % with respect to Kordiimonas. lacus S3-22(T) and Kordiimonas gwangyangensis JCM 12864(T), respectively. The genome-to-genome distance analysis revealed that strain M41(T) shared 51.4 % DNA-DNA relatedness with K. lacus S3-22(T) and 16.3 % with K. gwangyangensis JCM 12864(T). On the basis of phenotypic and genotypic characteristics, strain M41(T) represents a novel species of the genus Kordiimonas, for which the name Kordiimonas lipolytica sp. nov. is proposed. The type strain is M41(T) (=CGMCC 1.15304(T)=JCM 30877(T)). An emended description of Kordiimonas lacus is also provided.