Secretogranin II: a key AP-1-regulated protein that mediates neuronal differentiation and protection from nitric oxide-induced apoptosis of neuroblastoma cells

被引:22
作者
Li, L. [1 ]
Hung, A. C. [1 ]
Porter, A. G. [1 ]
机构
[1] Agcy Sci Technol & Res, Inst Mol & Cell Biol, Cell Death & Human Dis Grp, Div Canc & Dev Cell Grp, Singapore 138673, Singapore
关键词
nitric oxide; apoptosis; AP-1; secretogranin; neuronal differentiation;
D O I
10.1038/cdd.2008.8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Identification of AP-1 target genes in apoptosis and differentiation has proved elusive. Secretogranin II (SgII) is a protein widely distributed in nervous and endocrine tissues, and abundant in neuroendocrine granules. We addressed whether SgII is regulated by AP-1, and if SgII is involved in neuronal differentiation or the cellular response to nitrosative stress. Nitric oxide (NO) upregulated sgII mRNA dependent on a cyclic AMP response element (CRE) in the sgII promoter, and NO stimulated SgII protein secretion in neuroblastoma cells. Upregulation of sgII mRNA, sgII CRE-driven gene expression and SgII protein synthesis/export were attenuated in cells transformed with dominant-negative c-Jun (TAM67), which became sensitized to NO-induced apoptosis and failed to undergo nerve growth factor-dependent neuronal differentiation. Stable transformation of TAM67 cells with sgII restored neuronal differentiation and resistance to NO. RNAi knockdown of sgII in cells expressing functional c-Jun abolished neuronal differentiation and rendered the cells sensitive to NO-induced apoptosis. Therefore, SgII represents a key AP-1-regulated protein that counteracts NO toxicity and mediates neuronal differentiation of neuroblastoma cells.
引用
收藏
页码:879 / 888
页数:10
相关论文
共 38 条
  • [21] AP-1 function and regulation
    Karin, M
    Liu, ZG
    Zandi, E
    [J]. CURRENT OPINION IN CELL BIOLOGY, 1997, 9 (02) : 240 - 246
  • [22] The safety of aerosolized diethylenetriamine nitric oxide adduct after single-dose administration to anesthetized piglets and multiple-dose administration to conscious rats
    Lam, CF
    Caterina, P
    Filion, P
    Ilett, KF
    van Heerden, PV
    [J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 2003, 190 (01) : 65 - 71
  • [23] Two aerosolized nitric oxide adducts as selective pulmonary vasodilators for acute pulmonary hypertension
    Lam, CF
    van Heerden, PV
    Ilett, KF
    Caterina, P
    Filion, P
    [J]. CHEST, 2003, 123 (03) : 869 - 874
  • [24] EFFECTS OF NERVE GROWTH-FACTOR ON THE BIOSYNTHESIS OF CHROMOGRANIN-A AND CHROMOGRANIN-B, SECRETOGRANIN-II AND CARBOXYPEPTIDASE-H IN RAT PC12-CELLS
    LASLOP, A
    TSCHERNITZ, C
    [J]. NEUROSCIENCE, 1992, 49 (02) : 443 - 450
  • [25] Differential regulation of c-Jun by ERK and JNK during PC12 cell differentiation
    Leppä, S
    Saffrich, R
    Ansorge, W
    Bohmann, D
    [J]. EMBO JOURNAL, 1998, 17 (15) : 4404 - 4413
  • [26] Microarray analysis reveals an antioxidant responsive element-driven gene set involved in conferring protection from an oxidative stress-induced apoptosis in IMR-32 cells
    Li, J
    Lee, JM
    Johnson, JA
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (01) : 388 - 394
  • [27] Neuroendocrine cell type-specific and inducible expression of chromogranin/secretogranin genes
    Mahata, SK
    Mahapatra, NR
    Mahata, M
    O'Connor, DT
    [J]. CHROMAFFIN CELL: TRNSMITTER BIOSYNTHESIS, STORAGE, RELEASE, ACTIONS, AND INFORMATICS, 2002, 971 : 27 - 38
  • [28] Neuroendocrine cell type-specific and inducible expression of the secretogranin II gene: Crucial role of cyclic adenosine monophosphate and serum response elements
    Mahata, SK
    Mahata, M
    Livsey, CV
    Gerdes, HH
    Huttner, WB
    O'Connor, DT
    [J]. ENDOCRINOLOGY, 1999, 140 (02) : 739 - 749
  • [29] BCL-2 is upregulated in human SH-SY5Y neuroblastoma cells differentiated by overexpression of fibroblast growth factor 1
    Raguenez, G
    Desire, L
    Lantrua, V
    Courtois, Y
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 258 (03) : 745 - 751
  • [30] Isolation and characterization of the human secretogranin II gene promoter
    Scammell, JG
    Reddy, S
    Valentine, DL
    Coker, TN
    Nikolopoulos, SN
    Ross, RA
    [J]. MOLECULAR BRAIN RESEARCH, 2000, 75 (01): : 8 - 15