Kartogenin enhances the therapeutic effect of bone marrow mesenchymal stem cells derived exosomes in cartilage repair

被引：62
作者：
Liu, Chun [1 ]
Li, Yun [1 ]
Yang, Zhijian [2 ]
Zhou, Zhiyou [1 ]
Lou, Zhihao [1 ]
Zhang, Qiqing [1 ]
机构：
[1] Fuzhou Univ, Inst Biomed & Pharmaceut Technol, Fuzhou 350002, Peoples R China
[2] Fujian Agr & Forestry Univ, Coll Agr, Fuzhou 350002, Peoples R China
来源：
NANOMEDICINE | 2020年 / 15卷 / 03期
关键词：
bone marrow mesenchymal stem cells; chondrocytes; exosomes; kartogenin; osteoarthritis; OSTEOARTHRITIS; REGENERATION; CHONDROGENESIS; PROLIFERATION; ANGIOGENESIS; CHONDROCYTES; KNEE;
D O I：
10.2217/nnm-2019-0208
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effectiveness of mesenchymal stem cells (MSC) in the treatment of cartilage diseases has been demonstrated to be attributed to the paracrine mechanisms, especially the mediation of exosomes. But the exosomes derived from unsynchronized MSCs may be nonhomogeneous and the therapeutic effect varies between samples. Aim: To produce homogeneous and more effective exosomes for the regeneration of cartilage. Materials & methods: In this study we produced specific exosomes from bone marrow MSCs (BMSC) through kartogenin (KGN) preconditioning and investigated their performance in either in vitro or in vivo experiments. Results & conclusion: The exosomes derived from KGN-preconditioned BMSCs (KGN-BMSC-Exos) performed more effectively than the exosomes derived from BMSCs (BMSC-Exos). KGN preconditioning endowed BMSC-Exos with stronger chondral matrix formation and less degradation.
引用
收藏
页码：273 / 288
页数：16
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