Kartogenin enhances the therapeutic effect of bone marrow mesenchymal stem cells derived exosomes in cartilage repair

被引：62
作者：
Liu, Chun [1 ]
Li, Yun [1 ]
Yang, Zhijian [2 ]
Zhou, Zhiyou [1 ]
Lou, Zhihao [1 ]
Zhang, Qiqing [1 ]
机构：
[1] Fuzhou Univ, Inst Biomed & Pharmaceut Technol, Fuzhou 350002, Peoples R China
[2] Fujian Agr & Forestry Univ, Coll Agr, Fuzhou 350002, Peoples R China
来源：
NANOMEDICINE | 2020年 / 15卷 / 03期
关键词：
bone marrow mesenchymal stem cells; chondrocytes; exosomes; kartogenin; osteoarthritis; OSTEOARTHRITIS; REGENERATION; CHONDROGENESIS; PROLIFERATION; ANGIOGENESIS; CHONDROCYTES; KNEE;
D O I：
10.2217/nnm-2019-0208
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effectiveness of mesenchymal stem cells (MSC) in the treatment of cartilage diseases has been demonstrated to be attributed to the paracrine mechanisms, especially the mediation of exosomes. But the exosomes derived from unsynchronized MSCs may be nonhomogeneous and the therapeutic effect varies between samples. Aim: To produce homogeneous and more effective exosomes for the regeneration of cartilage. Materials & methods: In this study we produced specific exosomes from bone marrow MSCs (BMSC) through kartogenin (KGN) preconditioning and investigated their performance in either in vitro or in vivo experiments. Results & conclusion: The exosomes derived from KGN-preconditioned BMSCs (KGN-BMSC-Exos) performed more effectively than the exosomes derived from BMSCs (BMSC-Exos). KGN preconditioning endowed BMSC-Exos with stronger chondral matrix formation and less degradation.
引用
收藏
页码：273 / 288
页数：16
相关论文
共 38 条
  • [1] Comprehensive Proteomic Analysis of Mesenchymal Stem Cell Exosomes Reveals Modulation of Angiogenesis via Nuclear Factor-KappaB Signaling
    Anderson, Johnathon D.
    Johansson, Henrik J.
    Graham, Calvin S.
    Vesterlund, Mattias
    Pham, Missy T.
    Bramlett, Charles S.
    Montgomery, Elizabeth N.
    Mellema, Matt S.
    Bardini, Renee L.
    Contreras, Zelenia
    Hoon, Madeline
    Bauer, Gerhard
    Fink, Kyle D.
    Fury, Brian
    Hendrix, Kyle J.
    Chedin, Frederic
    El-Andaloussi, Samir
    Hwang, Billie
    Mulligan, Michael S.
    Lehtio, Janne
    Nolta, Jan A.
    [J]. STEM CELLS, 2016, 34 (03) : 601 - 613
  • [2] Freeze-dried and GMP-compliant pharmaceuticals containing exosomes for acellular mesenchymal stromal cell immunomodulant therapy
    Bari, Elia
    Perteghella, Sara
    Catenacci, Laura
    Sorlini, Marzio
    Croce, Stefania
    Mantelli, Melissa
    Avanzini, Maria A.
    Sorrenti, Milena
    Torre, Maria L.
    [J]. NANOMEDICINE, 2019, 14 (06) : 753 - 765
  • [3] Stem Cell-Derived Extracellular Vesicles as a Novel Potential Therapeutic Tool for Tissue Repair
    Chen, Bi
    Li, Qing
    Zhao, Bizeng
    Wang, Yang
    [J]. STEM CELLS TRANSLATIONAL MEDICINE, 2017, 6 (09) : 1753 - 1758
  • [4] Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles
    Colombo, Marina
    Raposo, Graca
    Thery, Clotilde
    [J]. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 30, 2014, 30 : 255 - 289
  • [5] Exosomes as Intercellular Signaling Organelles Involved in Health and Disease: Basic Science and Clinical Applications
    Corrado, Chiara
    Raimondo, Stefania
    Chiesi, Antonio
    Ciccia, Francesco
    De Leo, Giacomo
    Alessandro, Riccardo
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (03) : 5338 - 5366
  • [6] Mesenchymal stem cells derived exosomes and microparticles protect cartilage and bone from degradation in osteoarthritis
    Cosenza, Stella
    Ruiz, Maxime
    Toupet, Karine
    Jorgensen, Christian
    Noel, Daniele
    [J]. SCIENTIFIC REPORTS, 2017, 7
  • [7] The life and fate of mesenchymal stem cells
    Eggenhofer, Elke
    Luk, Franka
    Dahlke, Marc H.
    Hoogduijn, Martin J.
    [J]. FRONTIERS IN IMMUNOLOGY, 2014, 5
  • [8] Exosomes from Human Synovial-Derived Mesenchymal Stem Cells Prevent Glucocorticoid-Induced Osteonecrosis of the Femoral Head in the Rat
    Guo, Shang-Chun
    Tao, Shi-Cong
    Yin, Wen-Jing
    Qi, Xin
    Sheng, Jia-Gen
    Zhang, Chang-Qing
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2016, 12 (10): : 1262 - 1272
  • [9] Updated Projected Prevalence of Self-Reported Doctor-Diagnosed Arthritis and Arthritis-Attributable Activity Limitation Among US Adults, 2015-2040
    Hootman, Jennifer M.
    Helmick, Charles G.
    Barbour, Kamil E.
    Theis, Kristina A.
    Boring, Michael A.
    [J]. ARTHRITIS & RHEUMATOLOGY, 2016, 68 (07) : 1582 - 1587
  • [10] Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function
    Hu, Yin
    Rao, Shan-Shan
    Wang, Zhen-Xing
    Cao, Jia
    Tan, Yi-Juan
    Luo, Juan
    Li, Hong-Ming
    Zhang, Wei-She
    Chen, Chun-Yuan
    Xie, Hui
    [J]. THERANOSTICS, 2018, 8 (01): : 169 - 184
1234