Comparison of&IT Echinococcus multilocularis&IT and&IT Echinococcus granulosus &IThydatid fluid proteome provides molecular strategies for specialized host-parasite interactions

被引:29
作者
Ahn, Chun-Seob [1 ]
Kim, Jeong-Geun [1 ]
Han, Xiumin [2 ,3 ]
Kang, Insug [4 ]
Kong, Yoon [1 ]
机构
[1] Sungkyunkwan Univ, Samsung Med Ctr, Dept Mol Parasitol, Sch Med, Suwon, South Korea
[2] Qinghai Prov Inst Endem Dis Prevent & Control, Xining, Qinghai, Peoples R China
[3] Qinghai Prov Peoples Hosp, Clin Res Inst Hydatid Dis, Xining, Qinghai, Peoples R China
[4] Kyung Hee Univ, Dept Mol Biol & Biochem, Sch Med, Seoul, South Korea
关键词
alveolar echinococcosis; cystic echinococcosis; hydatid fluid; proteome; protein-protein interaction networks; ALVEOLAR ECHINOCOCCOSIS; CYSTIC ECHINOCOCCOSIS; EXPRESSION PATTERNS; HELMINTH PATHOGEN; HYDATID FLUID; ANTIGEN-B; PROTEINS; METACESTODE; DIFFERENTIATION; MAINTENANCE;
D O I
10.18632/oncotarget.20761
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Alveolar and cystic echinococcoses, caused by the metacestodes of Echinococcus multilocularis and E. granulosus, are prevalent in several regions and invoke deleterious zoonotic helminthiases. Hydatid fluid (HF), which contains proteinaceous and non-proteinaceous secretions of the parasite- and host-derived components, critically affects the host-parasite interplay and disease progression. We conducted HF proteome profiling of fully mature E. multilocularis vesicle (nine months postinfection) and E. granulosus cyst (stage 2). We identified 120 and 153 proteins, respectively, in each fluid. Fifty-six and 84 proteins represented distinct species; 44 and 66 were parasites, and 12 and 18 were host-derived proteins. The five major parasite protein populations, which included antigen B isoforms, metabolic enzymes, proteases and inhibitors, extracellular matrix molecules (ECMs), and developmental proteins, were abundantly distributed in both fluids and also exclusively in one sample or the other. Carbohydrate-metabolizing enzymes were enriched in E. granulosus HF. In the E. multilocularis HF, proteins that constitute ECMs, which might facilitate adhesion and cytogenesis, were highly expressed. Those molecules had physical and functional relationships along with their biochemical properties through protein-protein interaction networks. Twelve host-derived proteins were largely segregated to serum components. The major proteins commonly and uniquely detected in these HFs and their symbiotic interactome relationships might reflect their biological roles in similar but distinct modes of maturation, invasion, and the longevity of the parasites in the hosts.
引用
收藏
页码:97009 / 97024
页数:16
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