The Use of an In Vitro-cultured Porcine Nasal Mucosa Model for the Biocompatibility Assessment of Biodegradable Magnesium

被引：3
|
作者：
Schumacher, Stephan [1 ]
Stahl, Jessica [1 ]
Baeumer, Wolfgang [1 ]
Kietzmann, Manfred [1 ]
机构：
[1] Univ Vet Med Hannover, Dept Pharmacol Toxicol & Pharm, D-30559 Hannover, Germany
来源：
ATLA-ALTERNATIVES TO LABORATORY ANIMALS | 2011年 / 39卷 / 03期
关键词：
biocompatibility; CRS; degradation; magnesium; porcine nasal mucosa; tissue implant interface; ALLOYS; STENTS; PROLIFERATION; CORROSION; EXPLANTS; CALCIUM; METALS;
D O I：
10.1177/026119291103900312
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The development of an in vitro-cultured porcine nasal mucosa model is described. The model was subsequently used for the biocompatibility testing of resorbable magnesium-based implants, which are intended for use in the nasal cavity of patients with chronic rhinosinusitis (CRS). Test specimens made from either pure magnesium or titanium were incubated with the mucosal tissue for 48 hours. Afterwards, tissue viability, PGE(2), IL-6 and IL-8 release, magnesium ion release, succinate dehydrogenase activity, apoptosis and (14)C amino acid incorporation, were determined. The results suggested favourable biocompatibility, even in the case of rapidly-degrading pure magnesium. However, presumed effects on protein synthesis and apoptosis could not be confirmed.
引用
收藏
页码：261 / 271
页数：11
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